为了阐明质量碎片模式并揭示更多未描述的 ophiobolin 类似物，基于 UPLC-Q-TOF-MS/MS 实验对 ophiobolin 的质量碎片模式进行了分析。不同类型的重排（包括 McLafferty 重排）是主要的裂解模式。然后根据质量分析对二十六个 (9-31) 类似物进行了初步表征，并在目标中分离出了三种未描述的 ophiobolin (6-8) 和一个已知的类似物 (5)。化合物5具有最近才报道的罕见多环碳骨架，化合物6包含未描述的内酯环系统，其在C-3/C-21处与A/B环稠合，而化合物7和8在侧链中具有过氧基，这是所有蛇毒碱中首次报道的。化合物 5 和 7 对 MCF-7 癌细胞表现出显着的细胞毒性。版权所有 © 2024。由 Elsevier Ltd 出版。

In order to elucidate the mass fragmentation patterns and unveil more undescribed ophiobolin analogues, the mass fragmentation patterns of ophiobolins were analyzed based on UPLC-Q-TOF-MS/MS experiments. Different kinds of rearrangements (including McLafferty rearrangement) were the main cleavage patterns. Twenty-six (9-31) analogues were then tentatively characterized based on their mass analysis, and three undescribed ophiobolins (6-8) and a known analogue (5) were isolated in target. Compound 5 possesses a rare polycyclic carbon skeleton only recently reported, and compound 6 contains an undescribed lactone ring system fused with A/B ring at C-3/C-21, whereas compounds 7 and 8 have a peroxyl group in the side chain, which is the first reported in all ophiobolins. Compounds 5 and 7 displayed significant cytotoxicity against MCF-7 cancer cells.Copyright © 2024. Published by Elsevier Ltd.