目的 确定口腔面部肿瘤患者唾液和组织样本中 HPV 和疱疹病毒的患病率和关联性。对肿瘤进行活组织检查，并从口腔面部肿瘤患者收集唾液样本，用于使用巢式多重 PCR 测定病毒。自变量是性别、年龄、合并症、肿瘤分期和住院时间。结果变量是疱疹病毒和 HPV 的存在或不存在。进行了描述性总结和推论统计。该研究纳入了一百名患者。肿瘤中疱疹病毒和HPV的检出率分别为17.6%和57.0%，患者唾液中的检出率分别为48.3%和60.0%。检测到的疱疹病毒包括 EBV (21.3 %)、HHV-7 (11.2 %)、CMV (6.7 %)、HSV-1 (5.1 %)、HSV-2 (1.1 %)、VZV (1.1 %) 和卡波西肉瘤病毒 ( 0.6%）。最常见的 HPV 基因型是 HPV-42 (29%)、HPV-43 (22.7%)、HPV-52 (22.2%)、HPV-39 (18.8%) 和 HPV-18 (9.1%)。口腔面部恶性肿瘤中检出 EBV 的几率是口腔面部良性肿瘤的 2 倍。口面部肿瘤患者唾液中的 HPV DNA 为 69.7%，而对照样本为 18.2%（p < 0.001）。所有参与者的平均停留时间为 6.5 天，与病毒相关的人停留时间更长。口面部肿瘤患者的唾液和肿瘤样本中疱疹病毒和 HPV 的患病率很高，这表明在这方面还有可能开展更多工作区域。版权所有 © 2024 HCFMUSP。由 Elsevier España 出版，S.L.U.版权所有。

To determine the prevalence and association of HPV and Herpesviruses in saliva and tissue samples of patients with orofacial tumors.Biopsies of tumors were done, and saliva samples were collected from patients with orofacial tumors for the determination of viruses using nested multiplex PCR. Independent variables were sex, age, comorbidities, tumor stage, and length of stay. Outcome variables were the presence or absence of herpesviruses and HPV. Descriptive summaries and inferential statistics were done.A hundred patients were included in the study. Prevalence of herpesviruses and HPV were 17.6 % and 57.0 % in tumors, and 48.3 % and 60.0 % in the saliva of patients respectively. Herpesviruses detected included EBV (21.3 %), HHV-7 (11.2 %), CMV (6.7 %), HSV-1 (5.1 %), HSV-2 (1.1 %), VZV (1.1 %), and Kaposi sarcoma virus (0.6 %). The most prevalent HPV genotypes were HPV-42 (29 %), HPV-43 (22.7 %), HPV-52 (22.2 %), HPV-39 (18.8 %), and HPV-18 (9.1 %). The odds of EBV being detected in malignant orofacial tumors were 2 times that of benign orofacial tumors. HPV DNA in the saliva of patients with orofacial tumors was 69.7 %, compared to 18.2 % of the control sample (p < 0.001). The median length of stay for all participants was 6.5 days, those associated with viruses stayed longer.There was a high prevalence of Herpesviruses and HPV in saliva and tumor samples of patients with orofacial tumors, signalling some potential for more work to be done in this area.Copyright © 2024 HCFMUSP. Published by Elsevier España, S.L.U. All rights reserved.