创新的遗传剪刀策略及其在癌症治疗和预防中的应用：CRISPR模块和挑战
Innovative genetic scissor strategies and their applications in cancer treatment and prevention: CRISPR modules and challenges
影响因子:8.50000
分区:生物学2区 Top / 生化与分子生物学2区 应用化学2区 高分子科学2区
发表日期:2024 Nov
作者:
Sayedeh Azimeh Hosseini, Fatemeh Elahian, Seyed Abbas Mirzaei
摘要
有许多用于靶向基因组序列的基因编辑工具。有些几乎是已知的，大多数是一个完全的谜，没有发现。 CRISPR/CAS编辑工具带来了医学的重大革命。研究人员表明，与以前的方法相比，CRISPR可以更准确，经济和容易地修改DNA。 CRISPR已证明自己有效地将DNA片段的缺失，替代和插入到细胞类型，组织和生物体中。最近，将CRISPR/CAS与因子（转录因子/阻遏物，外核酸酶，转核酸酶，转骨，caspase，caspase，荧光蛋白，氧化蛋白，氧化酶，DNA/RNA聚合酶），DNA/RNA聚合酶）和元素（Aptamers，aptamers，barcodes，荧光探针，trigger trigge）具有转录的蛋白酶，蛋白酶的转录，蛋白酶，蛋白酶，蛋白酶，蛋白酶，蛋白酶，元素，这些模块正在接受预防和治疗的研究，本综述着重于这种创新组合，希望在不久的将来成为临床现实。
Abstract
There are lots of gene editing tools for targeting genome sequences. Some are almost known, and most are a complete mystery and undiscovered. CRISPR/Cas editing tools have brought about a major revolution in medicine. Researchers have shown that CRISPR can modify DNA much more accurately, economically and easily than previous methods. CRISPR has proven itself effective for the deletion, replacement and insertion of DNA fragments into cell types, tissues and organisms. Recently, combining CRISPR/Cas with factors (transcription factors/repressors, exonucleases, endonucleases, transposons, caspase, fluorescent proteins, oxidoreductive enzymes, DNA/RNA polymerases), and elements (aptamers, barcodes, fluorescent probes, Trigger) have provided genome, transcriptome, proteome and epigenome modification. These modules are being investigated for cancer prevention and therapy and this review focuses on such innovative combinations that hopefully will become a clinical reality in the near future.