创新基因剪刀策略及其在癌症治疗与预防中的应用：CRISPR模块与挑战
Innovative genetic scissor strategies and their applications in cancer treatment and prevention: CRISPR modules and challenges
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影响因子:8.5
分区:生物学2区 Top / 生化与分子生物学2区 应用化学2区 高分子科学2区
发表日期:2024 Nov
作者:
Sayedeh Azimeh Hosseini, Fatemeh Elahian, Seyed Abbas Mirzaei
DOI:
10.1016/j.ijbiomac.2024.135239
摘要
目前存在许多用于靶向基因组序列的基因编辑工具，其中一些已基本为人所知，而大多数仍是未知和未被发现的。CRISPR/Cas编辑工具在医学领域引发了重大革命。研究显示，CRISPR可以比以前的方法更准确、更经济、更简便地修饰DNA。CRISPR已被证实在删除、替换和插入DNA片段到细胞类型、组织和生物体中具有有效性。近年来，将CRISPR/Cas与转录因子/抑制子、外切酶、内切酶、转座子、半胱天冬酶、荧光蛋白、氧还原酶、DNA/RNA聚合酶等因素结合，以及适配体、条码、荧光探针、Trigger等元素，已实现基因组、转录组、蛋白质组和表观基因组的修饰。这些模块正被研究用于癌症预防和治疗，本综述重点介绍这些创新组合，并期待未来能成为临床实际应用的基础。
Abstract
There are lots of gene editing tools for targeting genome sequences. Some are almost known, and most are a complete mystery and undiscovered. CRISPR/Cas editing tools have brought about a major revolution in medicine. Researchers have shown that CRISPR can modify DNA much more accurately, economically and easily than previous methods. CRISPR has proven itself effective for the deletion, replacement and insertion of DNA fragments into cell types, tissues and organisms. Recently, combining CRISPR/Cas with factors (transcription factors/repressors, exonucleases, endonucleases, transposons, caspase, fluorescent proteins, oxidoreductive enzymes, DNA/RNA polymerases), and elements (aptamers, barcodes, fluorescent probes, Trigger) have provided genome, transcriptome, proteome and epigenome modification. These modules are being investigated for cancer prevention and therapy and this review focuses on such innovative combinations that hopefully will become a clinical reality in the near future.