阴道微生物群参与人乳头瘤病毒（HPV）感染和宫颈癌（CC）进展，而在此过程中阴道微生物组成的具体变化仍不清楚。本研究旨在观察不同宫颈部位阴道微生物组成的具体变化。本研究采用Illumina高通量基因测序技术测定16SrRNA的V4区，并观察不同宫颈病变部位的阴道微生物组成。 HPV感染和宫颈病变与正常人群有显着差异，但阴道微生物丰富度无显着差异。 CC患者阴道物种多样性高于高危HPV感染或CIN患者。主要表现为阴道微生物多样性增加，蓝藻、乳杆菌相对丰度下降，而嗜蛋白杆菌等杂菌相对丰度增加。正常女性、高危HPV患者和CC患者均存在特征性阴道生物标志物。具体而言，正常组的生物标志物为varibaculum，高危HPV组的生物标志物为糖多孢菌，CC组的生物标志物为变形杆菌、棒状杆菌、粪球菌、消化球菌和瘤胃球菌。不同宫颈病变的微生物是不同的。阴道微生物组成对健康女性、高危HPV感染患者以及宫颈病变有一定的诊断作用。这些微生物可能作为 CC 的潜在生物标志物。也为治疗HPV感染和宫颈病变提供了有效途径。版权所有©2024范韩范范曾赵。

Vaginal microbiota is involved in human papillomavirus (HPV) infection and cervical cancer (CC) progression, and the specific changes in vaginal microbial composition during this process remains uncertain.This study aimed to observe the changes in the specific composition of vaginal microorganisms in different cervical lesions and identify biomarkers at different stages of lesions.In this study we used the illumina high-throughput gene sequencing technology to determine the V4 region of 16SrRNA and observed the vaginal microbial composition in different cervical lesions.The vaginal microbiota of patients with high-risk HPV infection and cervical lesions is significantly different from that of the normal population, but there is no significant difference in the richness of vaginal microbes. The diversity of vaginal species in CC patients is higher than that in high-risk HPV infection or CIN patients. The main manifestation is an increase in the diversity of vaginal microbes, a decrease in the relative abundance of cyanobacteria and Lactobacillus, and an increase in the relative abundance of dialister, peptonephila and other miscellaneous bacteria. There are characteristic vaginal biomarker in normal women, high risk HPV patients and CC patients. In detail, the biomarker in the normal group was varibaculum, the biomarker in the high-risk HPV group was saccharopolyspora, the biomarker of the CC group was the Proteobacteria, Corynebacterium, Coprococcus, Peptococcus and Ruminococcus.The study indicated that the compositions of vaginal microbes in different cervical lesions is different. The vaginal microbial composition has a certain diagnostic effect on healthy women, patients with high-risk HPV infection and cervical lesions. These microbes may serve as potential biomarkers for CC. It also provided an effective way for the treatment of HPV infections and cervical lesions.Copyright © 2024 Fan, Han, Fan, Zeng and Zhao.