分析宫颈HPV感染，宫颈病变和阴道微生态之间的相关性
Analysis of the correlation between cervical HPV infection, cervical lesions and vaginal microecology
影响因子:4.80000
分区:医学2区 / 免疫学2区 微生物学2区
发表日期:2024
作者:
Zhongru Fan, Dongyu Han, Xin Fan, Yu Zeng, Lin Zhao
摘要
阴道菌群参与人类乳头瘤病毒（HPV）感染和宫颈癌（CC）的进展，在此过程中阴道微生物组成的特定变化仍然不确定。这项研究旨在观察不同颈椎病态和在不同阶段的阴道微生物的特定组成的变化，以确定不同阶段的生物量。测序技术确定16sRRNA的V4区域并观察到不同宫颈病变中阴道微生物组成。高危HPV感染和宫颈病变的阴道微生物群与正常群体的阴道微生物群明显不同，但是阴道微生物的丰富性没有显着差异。 CC患者阴道物种的多样性高于高危HPV感染或CIN患者的多样性。主要的表现是阴道微生物的多样性增加，蓝细菌和乳酸杆菌的相对丰度降低，以及对拨号剂，肽phe和其他杂项细菌的相对丰度的增加。正常妇女，高风险HPV患者和CC患者中有阴道生物标志物的特征性阴道生物标志物。详细介绍，正常组的生物标志物是瓦里利杆菌，高风险HPV组的生物标志物是糖浮游植物，CC组的生物标志物是proteebacteria，Corynebacterium，corynebacterium，coprococcus，coprocococcus，peptocococcus and Peptocococcus和uminococcus。阴道微生物组成对健康女性，高危HPV感染和宫颈病变具有一定的诊断作用。这些微生物可能是CC的潜在生物标志物。它还为治疗HPV感染和宫颈病变提供了有效的方法。
Abstract
Vaginal microbiota is involved in human papillomavirus (HPV) infection and cervical cancer (CC) progression, and the specific changes in vaginal microbial composition during this process remains uncertain.This study aimed to observe the changes in the specific composition of vaginal microorganisms in different cervical lesions and identify biomarkers at different stages of lesions.In this study we used the illumina high-throughput gene sequencing technology to determine the V4 region of 16SrRNA and observed the vaginal microbial composition in different cervical lesions.The vaginal microbiota of patients with high-risk HPV infection and cervical lesions is significantly different from that of the normal population, but there is no significant difference in the richness of vaginal microbes. The diversity of vaginal species in CC patients is higher than that in high-risk HPV infection or CIN patients. The main manifestation is an increase in the diversity of vaginal microbes, a decrease in the relative abundance of cyanobacteria and Lactobacillus, and an increase in the relative abundance of dialister, peptonephila and other miscellaneous bacteria. There are characteristic vaginal biomarker in normal women, high risk HPV patients and CC patients. In detail, the biomarker in the normal group was varibaculum, the biomarker in the high-risk HPV group was saccharopolyspora, the biomarker of the CC group was the Proteobacteria, Corynebacterium, Coprococcus, Peptococcus and Ruminococcus.The study indicated that the compositions of vaginal microbes in different cervical lesions is different. The vaginal microbial composition has a certain diagnostic effect on healthy women, patients with high-risk HPV infection and cervical lesions. These microbes may serve as potential biomarkers for CC. It also provided an effective way for the treatment of HPV infections and cervical lesions.