宫颈人乳头瘤病毒（HPV）感染、宫颈病变与阴道微生态相关性分析
Analysis of the correlation between cervical HPV infection, cervical lesions and vaginal microecology
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影响因子:4.8
分区:医学2区 / 免疫学2区 微生物学2区
发表日期:2024
作者:
Zhongru Fan, Dongyu Han, Xin Fan, Yu Zeng, Lin Zhao
DOI:
10.3389/fcimb.2024.1405789
摘要
阴道微生物群参与人乳头瘤病毒（HPV）感染及宫颈癌（CC）进展过程，但在此过程中阴道微生物组成的具体变化仍不明确。本研究旨在观察不同宫颈病变中阴道微生物的特定组成变化，并识别不同病变阶段的生物标志物。本研究采用Illumina高通量基因测序技术，针对16SrRNA的V4区域，观察不同宫颈病变的阴道微生物组成。结果显示，高危型HPV感染伴宫颈病变患者的阴道微生物与正常人群存在显著差异，但微生物丰富度无显著差异。宫颈癌（CC）患者的阴道微生物多样性高于高危型HPV感染或CIN患者。主要表现为阴道微生物多样性增加，蓝细菌和乳酸杆菌的相对丰度下降，而多联菌、肽霉菌属等杂菌的相对丰度增加。正常女性、高危型HPV患者及宫颈癌患者具有特征性的阴道生物标志物。具体而言，正常组的生物标志物为Varibaculum，高危型HPV组为Saccharopolyspora，宫颈癌组的生物标志物为变形菌门、棒状杆菌属、Coprococcus、Peptococcus和Ruminococcus。研究表明，不同宫颈病变的阴道微生物组成存在差异，微生物组成对健康女性、高危型HPV感染患者及宫颈病变的诊断具有一定的辅助作用。这些微生物可能成为宫颈癌的潜在生物标志物，同时为HPV感染和宫颈病变的治疗提供了有效途径。
Abstract
Vaginal microbiota is involved in human papillomavirus (HPV) infection and cervical cancer (CC) progression, and the specific changes in vaginal microbial composition during this process remains uncertain.This study aimed to observe the changes in the specific composition of vaginal microorganisms in different cervical lesions and identify biomarkers at different stages of lesions.In this study we used the illumina high-throughput gene sequencing technology to determine the V4 region of 16SrRNA and observed the vaginal microbial composition in different cervical lesions.The vaginal microbiota of patients with high-risk HPV infection and cervical lesions is significantly different from that of the normal population, but there is no significant difference in the richness of vaginal microbes. The diversity of vaginal species in CC patients is higher than that in high-risk HPV infection or CIN patients. The main manifestation is an increase in the diversity of vaginal microbes, a decrease in the relative abundance of cyanobacteria and Lactobacillus, and an increase in the relative abundance of dialister, peptonephila and other miscellaneous bacteria. There are characteristic vaginal biomarker in normal women, high risk HPV patients and CC patients. In detail, the biomarker in the normal group was varibaculum, the biomarker in the high-risk HPV group was saccharopolyspora, the biomarker of the CC group was the Proteobacteria, Corynebacterium, Coprococcus, Peptococcus and Ruminococcus.The study indicated that the compositions of vaginal microbes in different cervical lesions is different. The vaginal microbial composition has a certain diagnostic effect on healthy women, patients with high-risk HPV infection and cervical lesions. These microbes may serve as potential biomarkers for CC. It also provided an effective way for the treatment of HPV infections and cervical lesions.