微生物群落动态与包括癌症在内的许多疾病有关。人乳头瘤病毒独立的宫颈内腺癌 (HPVI ECA) 瘤间微生物群的多样性尚未得到充分表征。我们的目标是描绘 HPVI ECA 中的瘤内微生物群概况并研究其对肿瘤发生的潜在影响。我们分析了 45 例 HPVI ECA 病例，其中包括 36 例胃型 ECA (GEA) 和 9 种透明细胞癌 (CCC)。我们使用 5R-16S 核糖体 DNA 测序比较了癌性组织样本和邻近非癌性组织样本中的微生物组成。此外，我们研究了特定微生物与临床病理指标以及患者结果之间的相关性。我们的研究结果表明癌组织和邻近非癌组织之间的微生物谱存在显着差异。在 HPVI ECA 亚型中，GEA 比 CCC 表现出更多的微生物变异。使用随机森林算法，我们确定了两种不同的微生物特征，它们可以作为 HPVI ECA 的预测生物标志物并区分 GEA 和 CCC。不同的微生物丰度与 HPVI ECA 患者的临床特征相关。此外，高水平的微球菌和低水平的丛毛单胞菌科未知属75与 HPVI ECA 患者的较差预后相关。同样，微杆菌的丰富度与总生存期 (OS) 的降低相关，链球菌科微生物的大量存在与 GEA 患者的无复发生存期 (RFS) 的降低相关。有趣的是，高丰度的微球菌还与 GEA 患者较差的 OS 相关。该研究揭示了 HPVI ECA 中独特的微生物特征，这些特征有可能作为疾病预后的生物标志物。这些肿瘤相关微生物群特征与临床病理学特征之间的相关性强调了基于微生物组的干预措施的可能性。我们的研究为更深入地研究宫颈微生物组在 HPVI ECA 中的作用奠定了基础。版权所有 © 2024 Zhou、Chen、Lin、Zheng、Zhang 和 Zhou。

Microbial community dynamics have been involved in numerous diseases, including cancer. The diversity of intertumoral microbiota in human papillomavirus independent endocervical adenocarcinoma (HPVI ECA) is not well-characterized.Our objective is to delineate the intratumoral microbiota profile in HPVI ECA and investigate its potential influence on oncogenesis.We analyzed 45 HPVI ECA cases, comprising 36 gastric-type ECA (GEA) and 9 clear cell carcinomas (CCC). We compared the microbial composition within cancerous and adjacent noncancerous tissue samples using 5R-16S ribosomal DNA sequencing. Further, we investigated the correlation between specific microbes and clinical-pathological metrics as well as patient outcomes.Our findings demonstrate notable differences in the microbial spectra between cancerous and adjacent noncancerous tissues. Amongst HPVI ECA subtypes, GEAs exhibit more microbial variations compared to CCCs. Using the Random Forest algorithm, we identified two distinct microbial signatures that could act as predictive biomarkers for HPVI ECA and differentiate between GEA and CCC. Varied microbial abundances was related to clinical characteristics of HPVI ECA patients. In addition, high levels of Micrococcus and low levels of unknown genus75 from the Comamonadaceae family were associated with poorer outcomes in HPVI ECA patients. Similarly, an abundance of Microbacterium correlated with reduced overall survival (OS), and a high presence of Streptococcaceae family microbes was linked to reduced recurrence-free survival (RFS) in GEA patients. Intriguingly, a high abundance of Micrococcus was also associated with a worse OS in GEA patients.The study reveals distinct microbial signatures in HPVI ECA, which have potential as biomarkers for disease prognosis. The correlation between these tumor-associated microbiota features and clinicopathological characteristics underscores the possibility of microbiome-based interventions. Our research provides a foundation for more in-depth studies into the cervical microbiome's role in HPVI ECA.Copyright © 2024 Zhou, Chen, Lin, Zheng, Zhang and Zhou.