本研究旨在观察胃专安方对胃癌癌前病变(PLGC)大鼠的干预以及对胃黏膜微生物群和炎症因子的调节作用，探讨胃专安方的药效机制。进入空白对照组（BCG）；味专安方低、中、高剂量组（分别为LDG、MDG、HDG）；和自然恢复组（NRG）随机。中药组大鼠给予相应剂量的胃转安方，NRG组和卡介苗组大鼠给予等体积的蒸馏水，连续12周。之后采集大鼠胃黏膜标本，观察胃黏膜一般及病理变化；采用16S rDNA扩增子测序检测胃粘膜菌群变化，采用细胞因子抗体微阵列和Western blotting分析炎症因子。 PLGC大鼠的PLGC发生了显着的变化，而胃转安方有效地改善了它们，特别是在MDG和HDG方面（p < 0.05）。与NRG相比，乳酸菌、韦荣球菌等益生菌丰度增加，变形菌、假单胞菌等病原菌丰度减少（p < 0.05，p < 0.01），IL-2、IL-2相对含量降低。 4、胃粘膜中IL-13和MCP-1下降(p < 0.05)。此外，它还可以上调DNA结合转录调节因子、ABC型多药转运系统和相关酶，并显着影响病毒蛋白与细胞因子和细胞因子受体相互作用以及T细胞受体信号通路等信号通路（p < 0.05，p < 0.01)，可促进药物吸收利用，修复受损胃粘膜。研究证实胃转安方可通过调节胃粘膜菌群和炎症因子来治疗PLGC大鼠。版权所有©2024 卢刘尚毛孟和高。

This study aimed to observe the intervention of Weizhuan'an prescription on rats with precancerous lesions of gastric cancer (PLGC) as well as its regulation on gastric mucosal microflora and inflammatory factors and explore the pharmacodynamic mechanisms of Weizhuan'an Formula.The rats were classified into the blank control group (BCG); low-, medium-, and high-dose groups of Weizhuan'an prescription (LDG, MDG, and HDG, respectively); and natural recovery group (NRG) at random. The rats in the traditional Chinese medicine (TCM) group were given corresponding doses of Weizhuan'an formula, while the rats in the NRG and BCG were given an equivalent volume of distilled water for 12 weeks. After that, gastric mucosa samples of rats were collected to observe the general and pathological changes in the gastric mucosa; the changes in gastric mucosal microflora were detected by 16S rDNA amplicon sequencing, and the inflammatory factors were analyzed by cytokine antibody microarray and Western blotting.The results suggest that compared with the BCG, the pathology of gastric mucosa and gastric mucosal microflora and inflammatory factors in rats with PLGC have changed significantly, while Weizhuan'an formula effectively improved them, especially in the MDG and HDG (p < 0.05). Compared with the NRG, the abundance of probiotics such as Lactobacillus and Veillonella were increased, while the abundance of pathogens such as Proteobacteria and Pseudomonas was decreased (p < 0.05, p < 0.01), and the relative contents of IL-2, IL-4, IL-13, and MCP-1 in gastric mucosa were decreased (p < 0.05). Moreover, it can upregulate the DNA-binding transcriptional regulator, ABC type multidrug transport system, and related enzymes and affect the signaling pathways such as viral protein interaction with cytokine and cytokine receptor and T cell receptor signaling pathway significantly (p < 0.05, p < 0.01), which can promote drug absorption and utilization and repair damaged gastric mucosa.The study confirmed that Weizhuan'an prescription can treat rats with PLGC by regulating gastric mucosal microflora and inflammatory factors.Copyright © 2024 Lu, Liu, Shang, Mao, Meng and Gao.