3D 细胞培养是卵巢癌研究的基本工具，可以更有效地研究这种致命疾病的主要特征，包括高复发率和化疗耐药性。对这些现象的生物学基础进行更清晰、更全面的了解可以帮助开发更有效的治疗方法，从而改善患者的治疗结果。选择最合适的模型来研究与癌症相关的细胞生物学的不同方面具有挑战性，特别是因为可用于 3D 培养研究的检测方法尚未完全建立。为了最大限度地发挥卵巢癌 3D 细胞培养的实用性，我们对当前可用的模型进行了深入审查，同时考虑了每种方法以及用于评估结果的测定技术的优点和局限性。这种综合分析可以深入了解哪种模型检测对最适合研究卵巢癌生物学的不同参数，例如细胞增殖、基因表达或治疗反应。我们还描述了多个模型的组合使用如何可能成为复杂行为体外表征的最有效策略。©2024 Cortesi 等人。

3D cell cultures are a fundamental tool in ovarian cancer research that can enable more effective study of the main features of this lethal disease, including the high rates of recurrence and chemoresistance. A clearer, more comprehensive understanding of the biological underpinnings of these phenomena could aid the development of more effective treatments thus improving patient outcomes. Selecting the most appropriate model to investigate the different aspects of cell biology that are relevant to cancer is challenging, especially since the assays available for the study of 3D cultures are not fully established yet. To maximise the usefulness of 3D cell cultures of ovarian cancer, we undertook an in-depth review of the currently available models, taking into consideration the strengths and limitations of each approach and of the assay techniques used to evaluate the results. This integrated analysis provides insight into which model-assay pair is best suited to study different parameters of ovarian cancer biology such as cell proliferation, gene expression or treatment response. We also describe how the combined use of multiple models is likely to be the most effective strategy for the in vitro characterisation of complex behaviours.©2024 Cortesi et al.