本工作旨在探讨circ_ROBO1在鼻咽癌（NPC）中的功能。检测circ_ROBO1在鼻咽癌组织和细胞系中的表达。通过功能实验研究circ_ROBO1和/或miR-324-3p对鼻咽癌细胞增殖、迁移、侵袭和凋亡的调节。探讨了 circ_ROBO1、miR-324-3p 和 NME1 之间的相互作用。在小鼠中研究了肿瘤生长和转移。circ_ROBO1在NPC中过表达。 circ_ROBO1 的敲低抑制了 NPC 细胞的增殖、迁移和侵袭并诱导凋亡。 circ_ROBO1 的缺失减少了小鼠的肿瘤生长和转移。 circ_ROBO1 与 miR-324-3p 竞争上调 NME1。降低 miR-324-3p 表达会损害 circ_ROBO1 对 NPC 细胞的敲低效果。过表达的 circ_ROBO1 通过修改 miR-324-3p/NME1 轴促进 NPC 发育。© 2024。作者。

This work aims to explore circ_ROBO1's function in nasopharyngeal carcinoma (NPC).circ_ROBO1 expression in NPC tissues and cell lines was measured. The regulation of circ_ROBO1 and/or miR-324-3p on the proliferation, migration, invasion, and apoptosis of NPC cells was investigated by functional experiments. The interplay between circ_ROBO1, miR-324-3p, and NME1 was explored. Tumor growth and metastasis were studied in mice.circ_ROBO1 was overexpressed in NPC. Knockdown of circ_ROBO1 repressed proliferation, migration, and invasion and induced apoptosis of NPC cells. Loss of circ_ROBO1 reduced tumor growth and metastasis in mice. circ_ROBO1 competed with miR-324-3p to upregulate NME1. Lowering miR-324-3p expression impaired the effect of knockdown of circ_ROBO1on NPC cells.Overexpressed circ_ROBO1 promotes NPC development by modifying the miR-324-3p/NME1 axis.© 2024. The Author(s).