非洲猪瘟 (ASF) 是一种影响猪和野猪的病毒性疾病，给养猪业带来经济负担。在这项研究中，我们研究了删除 ASFV 多基因家族 110 (MGF110) 片段 (1 L-5-6 L) 的影响）对细胞凋亡调节和促炎细胞因子表达的影响。分析了被亲本“Volgograd/14c”菌株或基因缺失的“Volgograd/D(1L-5-6L) MGF110”菌株​​感染的猪外周血巨噬细胞中的基因表达。与基因删除毒株相比，感染 ASFV 亲本毒株的巨噬细胞中的 Caspase-3 活性高出 1.15 倍。 Caspase-3 (Cas-3)、干扰素-A (IFN-A)、肿瘤坏死因子 A (TNF-A)、B 细胞淋巴瘤-2 (Bcl-2)、核因子 Kappa B (NF) 的基因表达分析-kB)、白介素-12 (IL-12) 和热休克蛋白-70 (HSP-70) 在感染后的不同时间点使用 RT-qPCR 进行分析，结果显示菌株之间的表达谱存在显着差异。细胞因子（NF-kB 除外）的峰值表达发生在“Volgograd/D(1L-5-6L) MGF110”菌株​​感染后 24 小时。在感染 ASFV“Volgograd/14c”毒株的样本中，在 72 小时和 96 小时观察到最强烈的表达，但 Bcl-2 和 NF-kB 除外，它们在感染后 6 小时达到峰值。 “Volgograd/D(1L-5-6L) MGF110”菌株​​的细胞因子表达趋势更稳定，表达值更高。亲本菌株的表达趋势随着时间的推移而增加，在感染后 72 和 96 小时达到最大值，但总体表达水平低于基因缺失菌株。这些发现表明，删除多基因家族 110 个成员 (1 L-5-6 L) 有助于 ASFV 减毒，而不影响病毒复制动力学。© 2024。作者获得 Springer Nature B.V. 的独家许可。

African swine fever (ASF) is a viral disease that affects pigs and wild boars providing economic burden in swine industry.In this study, we investigated the effect of deleting the ASFV multigene family 110 (MGF110) fragment (1 L-5-6 L) on apoptosis modulation and the expression of proinflammatory cytokines. Gene expression in swine peripheral blood macrophages infected with either the parental "Volgograd/14c" strain or the gene-deleted "Volgograd/D(1L-5-6L) MGF110" strain was analyzed. Caspase-3 activity was 1.15 times higher in macrophages infected with the parental ASFV strain compared to the gene-deleted strain. Gene expression analysis of Caspase-3 (Cas-3), Interferon-A (IFN-A), Tumor Necrosis Factor A (TNF-A), B-cell Lymphoma-2 (Bcl-2), Nuclear Factor Kappa B (NF-kB), Interleukin-12 (IL-12), and Heat Shock Protein-70 (HSP-70) using RT-qPCR at various time points after infection revealed significant differences in expression profiles between the strains. The peak expression of cytokines (except NF-kB) occurred at 24 h post-infection with the "Volgograd/D(1L-5-6L) MGF110" strain. In samples infected with the ASFV "Volgograd/14c" strain, the most intense expression was observed at 72 and 96 h, except for Bcl-2 and NF-kB, which peaked at 6 h post-infection. The cytokine expression trend for the "Volgograd/D(1L-5-6L) MGF110" strain was more stable with higher expression values.The expression trend for the parental strain increased over time, reaching maximum values at 72 and 96 h post-infection, but the overall expression level was lower than that of the gene-deleted strain. These findings suggest that deleting the multigene family 110 members (1 L-5-6 L) contributes to ASFV attenuation without affecting virus replication kinetics.© 2024. The Author(s), under exclusive licence to Springer Nature B.V.