将人类自然杀伤细胞封装到新型明胶基聚合水凝胶网络中。
Encapsulation of human natural killer cells into novel gelatin-based polymeric hydrogel networks.
发表日期：2024 Sep 02
作者：
Sibel Cendere, Ceren Yuksel, Ercument Ovali, Beste Kinikoglu, Ozgul Gok
来源：
PHYSICAL THERAPY & REHABILITATION JOURNAL
摘要：
在先天免疫系统中，自然杀伤 (NK) 细胞是效应淋巴细胞，可通过限制疾病传播和组织损伤来控制多种肿瘤类型和微生物感染。 NK 具有肿瘤细胞杀伤能力，无需启动或事先激活，是潜在的抗癌疗法。在临床实践中，NK 用于静脉注射，因为它们通常以悬浮液形式生长，类似于其他血细胞。在这项研究中，我们设计了一种新型有效的基于生物材料的 NK 细胞递送平台，其中包括将 NK 细胞原位封装到三维 (3D) 生物相容性聚合物支架中，用于潜在的抗癌治疗。依靠海藻酸盐（ALG）聚合物和二价阳离子之间的物理交联，两种天然聚合物（明胶（GEL）和透明质酸（HA））渗透到孔隙中并生成具有改善的机械性能和稳定性的互穿水凝胶系统。在对水凝胶进行广泛表征后，使用我们的原位凝胶程序将 NK 细胞封装在内部，以提供仿生微环境。
In the innate immune system, natural killer (NK) cells are effector lymphocytes which control several tumor types and microbial infections by limiting disease spread and tissue damage. With tumor cell killing abilities, with no priming or prior activation, NKs are potential anti-cancer therapies. In clinical practice, NKs are used in intravenous injections as they typically grow as suspension, similar to other blood cells. In this study, we designed a novel and effective biomaterial-based platform for NK cell delivery, which included in-situ NK cell encapsulation into three-dimensional (3D) biocompatible polymeric scaffolds for potential anti-cancer treatments. Depending on physical cross-linking between an alginate (ALG) polymer and a divalent cation, two natural polymers (gelatin (GEL) and hyaluronic acid (HA)) penetrated into pores and generated an inter-penetrating hydrogel system with improved mechanical properties and stability. After extensive characterization of hydrogels, NK cells were encapsulated inside using our in-situ gelation procedure to provide a biomimetic microenvironment.
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