使用核桃壳绿色合成氧化铜纳米粒子及其对乳腺癌和结直肠癌细胞系的尺寸依赖性抗癌作用。
Green synthesis of copper oxide nanoparticles using walnut shell and their size dependent anticancer effects on breast and colorectal cancer cell lines.
发表日期：2024 Sep 02
作者：
Hanieh Abdollahzadeh, Yaghub Pazhang, Asghar Zamani, Yousef Sharafi
来源：
ANTIOXIDANTS & REDOX SIGNALING
摘要：
金属氧化物纳米粒子（NP）具有独特的性质，这使其成为癌症治疗中有吸引力的药物。 CuO纳米粒子是使用核桃壳粉末在不同的煅烧温度（400°、500°、700°和900°C）下绿色合成的。 CuO 纳米粒子通过 FTIR、XRD、BET、SEM 和 DLS 分析进行表征。 SEM 和 DLS 分析表明，通过提高合成纳米颗粒所需的煅烧温度，它们的尺寸会增加。 DPPH 分析显示 CuO 纳米颗粒没有显着的抗氧化特性。 MTT分析显示所有合成的CuO NPs对MCF-7、HCT-116和HEK-293细胞系均表现出细胞毒作用。在CuO NPs中，CuO-900 NPs对HEK-293细胞系的细胞毒性作用最小(IC50 = 330.8 µg/ml)。 Hoechst 染色和实时分析表明 CuO-900 NP 通过提高 p53 和 Bax 基因表达水平来诱导细胞凋亡。此外，尽管在 HCT-116 细胞中，CuO-900 NP 仍增加了 MCF-7 细胞中的 Nrf-2 基因表达水平。从结果中可以得出结论，CuO-900 NP 对乳腺癌和结肠癌细胞具有良好的细胞毒性作用。© 2024。作者。
Metal oxide nanoparticles(NPs) contain unique properties which have made them attractive agents in cancer treatment. The CuO nanoparticles were green synthesized using walnut shell powder in different calcination temperatures (400°, 500°, 700°, and 900 °C). The CuO nanoparticles are characterized by FTIR, XRD, BET, SEM and DLS analyses. SEM and DLS analyses showed that by increasing the required calcination temperature for synthesizing the NPs, their size was increased. DPPH analysis displayed no significant anti-oxidative properties of the CuO NPs. The MTT analysis showed that all synthesized CuO NPs exhibited cytotoxic effects on MCF-7, HCT-116, and HEK-293 cell lines. Among the CuO NPs, the CuO-900 NPs showed the least cytotoxic effect on the HEK-293 cell line (IC50 = 330.8 µg/ml). Hoechst staining and real-time analysis suggested that the CuO-900 NPs induced apoptosis by elevation of p53 and Bax genes expression levels. Also, the CuO-900 NPs increased the Nrf-2 gene expression level in MCF-7 cells, despite the HCT-116 cells. As can be concluded from the results, the CuO-900 NPs exerted promising cytotoxic effects on breast and colon cancer cells.© 2024. The Author(s).