白血病抑制因子（LIF）参与不同癌症的进展。在这项研究中，我们研究了抗 LIF 抗体对 Balb/c 乳腺癌小鼠模型中免疫相关基因表达的影响。为了对小鼠进行 LIF 免疫，将含有弗氏佐剂的重组 LIF 注射到试验组中，而对照组则注射含有佐剂的磷酸盐缓冲盐水。通过增加抗体滴度进行肿瘤诱导（4T1细胞系）。通过实时PCR评估免疫相关基因的表达。免疫组的抗-LIF效价显着增加。免疫组中与辅助性T细胞（Th）-1、Th-2和Th-17细胞分化相关的基因表达量显着高于对照组。此外，抗LIF对调节性T细胞分化相关基因和免疫检查点相关基因的表达没有显着影响。此外，测试组的存活率更高，肿瘤发生率更低。结果表明，抗LIF抗体可能在免疫细胞分化或免疫反应中发挥作用。然而，需要利用先进技术进行进一步研究来验证其功能。© 2024。作者。

Leukemia inhibitory factor (LIF) is involved in the progression of different cancers. In this study, we investigated the effect of anti-LIF antibodies on immune-related gene expression in the Balb/c mouse model of breast cancer. To immunize mice against LIF, recombinant LIF with Freund adjuvant was injected into the test group, whereas the control group received phosphate-buffered saline with adjuvant. Tumor induction (4T1 cell line) was performed by increasing the antibody titer. The expression of immune-related genes was evaluated by real-time PCR. The anti-LIF titer was significantly increased in the immunized group. The expression of genes related to the differentiation of T helper (Th)-1, Th-2, and Th-17 cells was significantly higher in the immunized group than in the control group. In addition, anti-LIF did not have a significant effect on the expression of genes related to the differentiation of regulatory T cells, and immune checkpoint-associated genes. Additionally, the test group had higher survival and lower tumor development rates. The results demonstrated that the anti-LIF antibody may potentially play a role in the differentiation of immune cells or immune responses. However, further studies utilizing advanced techniques are necessary to validate its function.© 2024. The Author(s).