岩藻甾醇因其抗氧化、抗癌和抗炎特性而被广泛研究。然而，它们尚未在牙科领域得到研究。本研究旨在确定树脂修复前用岩藻甾醇对牙本质进行预处理是否可以增强树脂-牙本质混合层的粘合稳定性。将 0.1、0.5 和 1.0 wt% 岩藻甾醇应用于脱矿牙本质后，在胶原酶老化前后进行微拉伸粘合强度 (MTBS) 和纳米渗漏测试，并使用扫描电子显微镜 (SEM) 观察表面。岩藻甾醇处理组在胶原酶老化前后均表现出更好的粘合强度和更少的纳米渗漏，并且使用SEM确认了相应的结构。 MMP 酶谱证实，沿着岩藻甾醇浓度梯度，MMP 的活性相对较低，FTIR 分析证实了胶原交联的产生。此外，岩藻甾醇对变形链球菌（蛀牙的主要原因）具有细胞毒性。这项研究的结果表明，岩藻甾醇预处理可提高粘合强度并减少树脂-牙本质界面处的纳米渗漏，这可能是通过抑制内源性和外源性 MMP 活性而形成胶原交联的机制。这项研究证明了岩藻甾醇作为牙本质 MMP 抑制剂的潜力，有助于树脂-牙本质粘合的长期稳定性，并可用作修复材料。© 2024。作者。

Fucosterols have been widely studied for their antioxidant, anticancer, and anti-inflammatory properties. However, they have not yet been studied in the field of dentistry. This study aimed to determine whether pretreatment of dentin with fucosterol before resin restoration enhances bond stability in resin-dentin hybrid layers. After applying 0.1, 0.5, and 1.0 wt% fucosterol to demineralized dentin, microtensile bond strength (MTBS) and nanoleakage tests were performed before and after collagenase aging, and the surface was observed using scanning electron microscope (SEM). The fucosterol-treated group showed better bond strength and less nanoleakage both before and after collagenase aging, and the corresponding structures were confirmed using SEM. MMP zymography confirmed that the activity of MMPs was relatively low along the concentration gradient of fucosterol, and the FTIR analysis confirmed the production of collagen crosslinks. In addition, fucosterol exhibits cytotoxicity against Streptococcus mutans, the main cause of dental decay. The results of this study suggest that fucosterol pretreatment improves bond strength and reduces nanoleakage at the resin-dentin interface, possibly through a mechanism involving collagen cross-link formation via the inhibition of endogenous and exogenous MMP activity. This study demonstrates the potential of fucosterol as an MMP inhibitor in dentin, which contributes to long-term resin-dentin bond stability and can be used as a restorative material.© 2024. The Author(s).