切割和聚腺苷酸化因子是脂肪生成的潜在调节因子。
Cleavage and polyadenylation factors are potential regulators of adipogenesis.
发表日期：2024 Sep 02
作者：
Salwa Mohd Mostafa, Claire Moore
来源：
Cellular & Molecular Immunology
摘要：
选择性多聚腺苷酸化 (APA) 是一种共转录过程，可导致 mRNA 3' 端异构体多样性。已知 APA 发生在分化过程中，并且在癌症和自身免疫性疾病等疾病中观察到其失调。此前有报道称，3T3-L1细胞分化为脂肪细胞会导致mRNA整体延长，但参与这种调节的蛋白质尚未确定。裂解和聚腺苷酸化 (C/P) 复合物亚基的表达水平可以调节 Poly(A) 位点的选择，进而影响不同的细胞活性。在本文中，我们研究了3T3-L1分化过程中C/P蛋白水平的变化。我们观察到，虽然这些蛋白的RNA表达在分化过程中没有变化，但某些亚基的蛋白水平确实发生了变化，包括水平下降CPSF73，一种在 Poly(A) 位点切割的核酸酶。然而，单独过度表达 CPSF73 并不影响分化的效率和速率。© 2024。作者。
Alternative polyadenylation (APA) is a co-transcriptional process that leads to isoform diversity in the 3' ends of mRNAs. APA is known to occur during differentiation, and its dysregulation is observed in diseases like cancer and autoimmune disorders. It has been previously reported that differentiation of 3T3-L1 cells to adipocytes leads to an overall lengthening of mRNAs, but the proteins involved in this regulation have not been identified. The expression levels of subunits of the cleavage and polyadenylation (C/P) complex can regulate the choice of poly(A) site, which in turn can affect different cellular activities. In this paper, we studied the change in levels of C/P proteins during 3T3-L1 differentiation.We observed that while the RNA expression of these proteins is unchanged during differentiation, the protein levels of some subunits do change, including a decrease in levels of CPSF73, the nuclease that cuts at the poly(A) site. However, overexpression of CPSF73 alone does not affect the efficiency and rate of differentiation.© 2024. The Author(s).