全球对生殖健康的关注不断升级，凸显了调查环境污染物对生育力影响的紧迫性。本研究旨在关注广泛使用的有机磷杀虫剂毒死蜱（CPF），探讨其对Wistar雄性大鼠下丘脑-垂体-睾丸轴的不利影响。本研究探讨了白杨素纳米晶体（CHN）（一种已知具有抗氧化和抗炎特性的黄酮类化合物）对雄性 Wistar 大鼠 CPF 引起的损伤的潜在保护作用。采用溶剂沉淀法制备白杨素纳米晶体。六组雄性 Wistar 大鼠接受 30 天的治疗，包括对照组、单独用 CPF 治疗组、用 5 mg/kg 和 10 mg/kg 剂量的 CHN 治疗组、以及与 CPF 联合治疗组和中国。评估了生殖激素、睾丸功能酶生物标志物、氧化应激和炎症生物标志物的血清水平。此外，还对下丘脑、睾丸和附睾进行了组织学检查。CHN 具有抗氧化和抗炎特性，可有效抵消 CPF 引起的黄体生成素 (LH)、血清睾酮、卵泡刺激素 (FSH) 和睾丸的降低。酶生物标志物。此外，CHN 增强了抗氧化防御，下丘脑、睾丸、附睾中的丙二醛 (MDA) 水平降低和谷胱甘肽 (GSH) 水平增加就证明了这一点。与仅 CPF 组相比，CHN 联合治疗组的炎症标志物，包括一氧化氮 (NO)、白介素 6 (IL-6) 和肿瘤坏死因子 α (TNF-α) 显着降低。组织病理学分析证实了 CHN 对组织完整性的保护作用。白杨素纳米晶体通过其抗炎和抗氧化特性，在缓解 CPF 诱导的雄性大鼠生殖缺陷方面具有良好的潜力。这项研究为针对环境毒素引起的生殖毒性的治疗干预提供了宝贵的见解，强调了白杨素纳米晶体作为 CPF 暴露背景下的保护剂的潜力。版权所有 © Bentham Science Publishers；如有任何疑问，请发送电子邮件至 epub@benthamscience.net。

The escalating global concerns regarding reproductive health underscore the urgency of investigating the impact of environmental pollutants on fertility. This study aims to focus on Chlorpyrifos (CPF), a widely-used organophosphate insecticide, and explores its adverse influence on the hypothalamic-pituitary-testicular axis in Wistar male rats. This study explores the potential protective effects of chrysin nanocrystal (CHN), a flavonoid with known antioxidant and anti-inflammatory properties, against CPF-induced impairments in male Wistar rats.Chrysin nanocrystals were prepared using a solvent precipitation method. Six sets of male Wistar rats were subjected to 30 days of treatment, comprising a control group, a group treated solely with CPF, groups treated with CHN at doses of 5 mg/kg and 10 mg/kg, and groups co-treated with CPF and CHN. Serum levels of reproductive hormones, enzyme biomarkers of testicular function, oxidative stress, and inflammatory biomarkers were assessed. Additionally, histological examinations were conducted on the hypothalamus, testes, and epididymis.CHN exhibited antioxidant and anti-inflammatory properties, effectively counteracting CPF-induced reductions in Luteinizing Hormone (LH), serum testosterone, Follicle-Stimulating Hormone (FSH), and testicular enzyme biomarkers. Moreover, CHN enhanced antioxidant defenses, as evidenced by decreased malondialdehyde (MDA) and increased glutathione (GSH) levels in the hypothalamus, and testes, epididymis. Inflammatory markers, including nitric oxide (NO), interleukin-6 (IL-6), and tumor necrosis factor-alpha (TNF-α), were significantly reduced in CHN co-treated groups compared to the CPF-only group. Histopathological analyses confirmed the protective effects of CHN on tissue integrity.Chrysin nanocrystal demonstrated promising potential in mitigating CPF-induced reproductive deficits in male rats through its anti-inflammatory and antioxidant properties. This study provides valuable insights into therapeutic interventions against environmental toxin-induced reproductive toxicity, emphasizing the potential of chrysin nanocrystals as a protective agent in the context of CPF exposure.Copyright© Bentham Science Publishers; For any queries, please email at epub@benthamscience.net.