使用凋亡生物标志物探讨 3 种羊肚菌提取物（圆锥羊肚菌、羊肚菌和美味羊肚菌）对乳腺癌和结肠癌细胞系的潜在凋亡机制。人乳腺癌细胞系 (MCF-7) 和结肠癌细胞系 (SW-480)用 3 种羊肚菌的甲醇和乙醇提取物进行处理，浓度范围为 0.0625 至 2 mg/mL。然后采用逆转录聚合酶链式反应测定它们对凋亡相关标志物（促凋亡标志物包括Bax、caspase-3、caspase-7和caspase-9，以及抗凋亡标志物包括Bcl-2）基因表达的影响。所有羊肚菌提取物在半数抑制浓度 (IC50) 为 0.02 ±0.01 至 0.68 ±0.30 mg/mL 时均能减少乳腺癌和结肠癌细胞的增殖。正如预期的那样，所有羊肚菌提取物均显着增加 MCF-7 和 SW-480 细胞系中 Bax、caspase-3、caspase-7 和 caspase-9 的基因表达，并下调 Bcl-2 的基因表达（P<0.05）。羊肚菌提取物通过凋亡诱导机制对乳腺癌和结肠癌细胞系表现出显着的抗增殖活性。羊肚菌提取物的抗癌活性以及对乳腺癌和结肠癌细胞凋亡的激活表明，未来它可用于开发抗癌化疗药物。© 2024。中华中西医结合杂志和德国施普林格出版社，施普林格自然的一部分。

To explore the potential apoptotic mechanisms of 3 Morchella extracts (Morchella conica, Morchella esculenta and Morchella delicosa) on breast and colon cancer cell lines using apoptotic biomarkers.Human breast cell line (MCF-7) and colon cancer cell line (SW-480) were treated with methanol and ethanol extracts of 3 Morchella species with concentration ranging from 0.0625 to 2 mg/mL. After that their effects on gene expression of apoptosis related markers (pro-apoptotic markers including Bax, caspase-3, caspase-7, and caspase-9, and the antiapoptotic marker including Bcl-2) were determined using reverse transcription polymerase chain reaction.All Morchella extracts reduced breast and colon cancer cells proliferation at half inhibitory concentration (IC50) of 0.02 ±0.01 to 0.68 ±0.30 mg/mL. As expected, all Morchella extracts significantly increased gene expressions of Bax, caspase-3, caspase-7, and caspase-9 and downregulated the gene expression of Bcl-2 in MCF-7 and SW-480 cell lines (P<0.05).Morchella extracts demonstrated significant anti-proliferative activity against breast and colon cancer cell lines via an apoptosis induction mechanism. Anticancer activity of Morchella extracts and activation of apoptosis in breast and colon cancer cells suggest that it may be used to develop chemotherapeutic agents against cancer in future.© 2024. The Chinese Journal of Integrated Traditional and Western Medicine Press and Springer-Verlag GmbH Germany, part of Springer Nature.