探讨和验证鸦胆子油乳注射液（YDZI）和生脉注射液（SMI）治疗胃癌（GC）时外周微循环障碍的疗效及潜在机制。通过网络药理学探讨YDZI和SMI的潜在机制并通过细胞和临床实验得到验证。培养人微血管内皮细胞（HMEC）进行实时定量聚合酶链反应、蛋白质印迹分析，培养人脐静脉内皮细胞（HUVEC）进行成管实验。招募了 20 名健康志愿者和 97 名 GC 患者。将患者分为手术切除组、手术切除联合化疗组、手术切除联合化疗联合YDZI组和SMI组。通过激光散斑对比成像结合闭塞后反应性充血来测量和记录前臂皮肤血液灌注。计算并比较各组皮肤血管电导率和微血管反应性参数。经过网络药理学分析，筛选出YDZI和SMI中的4个成分、82个活性成分和92个相关基因。 YDZI 和 SMI 的活性成分和交叉化合物 β-谷甾醇，上调血管内皮生长因子 A (VEGFA) 和前列腺素内过氧化物合酶 2 (PTGS2，P<0.01) 的表达，下调 caspase 9 (CASP9) 的表达奥沙利铂刺激下HMECs中雌激素受体1（ESR1，P<0.01），并通过VEGFA促进管形成。化疗显着损害GC患者的微血管反应性，而YDZI和SMI改善这种损伤（P<0.05或P<0.01）。YDZI和SMI改善GC患者的外周微血管反应性。 β-谷甾醇可通过调节 VEGFA、PTGS2、ESR1 和 CASP9 改善外周微循环。© 2024。中华中西医结合杂志出版社和德国 Springer-Verlag GmbH（Springer Nature 旗下公司）。

To explore and verify the effect and potential mechanism of Brucea javanica Seed Oil Emulsion Injection (YDZI) and Shengmai Injection (SMI) on peripheral microcirculation dysfunction in treatment of gastric cancer (GC).The potential mechanisms of YDZI and SMI were explored through network pharmacology and verified by cellular and clinical experiments. Human microvascular endothelial cells (HMECs) were cultured for quantitative real-time polymerase chain reaction, Western blot analysis, and human umbilical vein endothelial cells (HUVECs) were cultured for tube formation assay. Twenty healthy volunteers and 97 patients with GC were enrolled. Patients were divided into surgical resection, surgical resection with chemotherapy, and surgical resection with chemotherapy combining YDZI and SMI groups. Forearm skin blood perfusion was measured and recorded by laser speckle contrast imaging coupled with post-occlusive reactive hyperemia. Cutaneous vascular conductance and microvascular reactivity parameters were calculated and compared across the groups.After network pharmacology analysis, 4 ingredients, 82 active compounds, and 92 related genes in YDZI and SMI were screened out. β-Sitosterol, an active ingredient and intersection compound of YDZI and SMI, upregulated the expression of vascular endothelial growth factor A (VEGFA) and prostaglandin-endoperoxide synthase 2 (PTGS2, P<0.01), downregulated the expression of caspase 9 (CASP9) and estrogen receptor 1 (ESR1, P<0.01) in HMECs under oxaliplatin stimulation, and promoted tube formation through VEGFA. Chemotherapy significantly impaired the microvascular reactivity in GC patients, whereas YDZI and SMI ameliorated this injury (P<0.05 or P<0.01).YDZI and SMI ameliorated peripheral microvascular reactivity in GC patients. β-Sitosterol may improve peripheral microcirculation by regulating VEGFA, PTGS2, ESR1, and CASP9.© 2024. The Chinese Journal of Integrated Traditional and Western Medicine Press and Springer-Verlag GmbH Germany, part of Springer Nature.