目的目前的研究工作主要是通过针对RhoA基因和细胞凋亡途径基因和蛋白进行抗增殖试验来评价沙棘和沙棘Fe2O3纳米颗粒对肝癌细胞系(HepG2)的作用。方法利用沙棘提取物合成Fe2O3纳米颗粒。鼠李糖和 C. intybus 并通过紫外-可见光谱、FTIR、SEM/EDS 和 XRD 进行表征。 MTT法用于研究对HepG2细胞的细胞毒性。采用实时qPCR和ELISA对基因和蛋白进行分析。结果紫外-可见分光光度法观察到沙棘纳米粒在300 nm处有吸收峰，C. intybus纳米颗粒在289 nm处有吸收峰。 H. rhamnoide Fe2O3 NPs 的 FTIR 谱带表明存在醛、醇和多元醇，而 C. intybus Fe2O3 NPs 的谱带表明存在羧基、羟基、炔烃和胺。研究发现，H. rhamnoides 的 Fe2O3 纳米颗粒尺寸为 27±±5 nm，C. intybus 的 Fe2O3 纳米颗粒尺寸为 84±±4 nm。与植物提取物相比，H. rhmnoides 和 C. intybus Fe2O3 NPs 对 HepG2 细胞的 IC50 值为 41.69 µM 和 71.04 µM（H. rhamnoides 和 C. intybus 分别为 78.10 µM 和 96.03 µM）。 RhoA 的基因表达和蛋白水平下降，而 bax、caspase,3、caspase, 8 和 caspase 9 的基因表达和蛋白水平增加。 结论 与 C. intybus 相比，纳米颗粒和沙棘提取物更有效，这一点通过细胞毒性结果以及研究基因和蛋白质的分析。

ObjectiveThe current research work focused on the evaluation of of H. rhamnoides and C. intybus Fe2O3 NPs against liver cancer cell line (HepG2) by performing antiproliferative assay targeting the RhoA gene and apoptotic pathway genes and proteins.MethodsFe2O3 NPs were synthesized using extracts of H. rhamnoides and C. intybus and characterized by UV-Vis spectroscopy, FTIR, SEM/EDS and XRD. MTT assay was used to study cytotoxicity against the HepG2 cells. Real-time qPCR and ELISA were used for the gene and protein analysis.ResultsAn absorbance peak at 300 nm for H. rhamnoides and 289 nm for C. intybus nanoparticles were observed by UV-Vis analysis. The FTIR bands of H. rhamnoide Fe2O3 NPs suggested the presence of aldehydes, alcohols and polyols whereas bands of C. intybus Fe2O3 NPs suggested the presence of carboxyl groups, hydroxyl groups, alkynes and amines. The size of Fe2O3 NPs was found to be 27 ± 5nm for H. rhamnoides and 84 ± 4nm for C. intybus. The IC50 value of 41.69 µM for H. rhmnoides and 71.04 µM for C. intybus Fe2O3 NPs compared to plant extract (78.10 µM and 96.03 µM for H. rhamnoides and C. intybus respectively) were found against HepG2 cells. The gene expression and protein levels of RhoA were decreased whereas those of bax, caspase,3, caspase, 8 and caspase 9 were found increased.ConclusionNanoparticles and extract of H. rhamnoides were found more effective as compared to C. intybus which was evident by the results of cytotoxicity and analysis of studied genes and proteins.