由于其抗氧化作用，类胡萝卜素番茄红素已被证明在多种疾病模型中具有保护作用；然而，其对纳米二氧化钛 (nano-TiO2) 引起的神经毒性的影响尚未确定。本研究的目的是评估番茄红素如何影响纳米二氧化钛引起的神经元损伤及其机制。 SH-SY5Y细胞用不同浓度的纳米TiO2处理48小时，然后使用MTT（3-（4,5-二甲基噻唑-2-基）-2,5-二苯基溴化四唑）试验评估细胞活力。测定了纳米 TiO2 的 IC50，结果显示 IC50 等于 40μM/mL，在暴露于 40μM 纳米 TiO2 之前一小时，将番茄红素 (10μM) 应用于 SH-SY5Y 人神经母细胞瘤细胞。测量活性氧、脂质过氧化、一氧化氮、谷胱甘肽、超氧化物歧化酶和过氧化氢酶、肿瘤坏死因子-α、白细胞介素 1β、核因子 kappa B 和凋亡标记物（Bcl2、Bax 和 caspase-3）确定番茄红素的抗氧化作用。根据 MTT 测定结果和乳酸脱氢酶 (LDH) 细胞毒性评估，在 SH-SY5Y 神经母细胞瘤细胞中，用 10 µM 番茄红素预处理可显着降低暴露于纳米 TiO2 所带来的毒性。在暴露于纳米二氧化钛的细胞中，番茄红素预处理显着提高了抗氧化酶的活性并减少了氧化应激。此外，当 SH-SY5Y 细胞接受纳米 TiO2 处理时，番茄红素预处理可阻止神经炎症和细胞凋亡。这项研究的结果表明，番茄红素可能是一种有效的神经保护剂，可对抗氧化应激和神经炎症，并可用于阻止各种神经系统疾病中的神经元死亡或损伤。

Due to its antioxidant action, the carotenoid lycopene has been demonstrated to have a protective effect in several disease models; however, its effect on the nanoform of titanium oxide (nano-TiO2)-induced neurotoxicity has not yet been determined. The purpose of this study was to evaluate how lycopene affects neuronal damage brought on by nano-TiO2 and the mechanisms involved. SH-SY5Y cells were treated with different concentrations of nano-TiO2 for 48 hours, the MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) test was used after that to evaluate cell viability. IC50 of nano-TiO2 was determined and the results revealed that IC50 is equal 40 µM/mL, lycopene (10 µM) was applied to SH-SY5Y human neuroblastoma cells an hour before exposure to 40 µM nano-TiO2. Reactive oxygen species, lipid peroxidation, nitric oxide, glutathione, superoxide dismutase, and catalase, tumor necrosis factor-alpha, interleukin 1 beta, nuclear factor kappa B, and apoptotic markers (Bcl2, Bax, and caspase-3), were measured to determine the anti-oxidant effect of lycopene. In SH-SY5Y neuroblastoma cells, pretreatment with 10 µM lycopene significantly reduced the toxicity brought on by exposure to nano-TiO2, according to MTT assay findings and lactate dehydrogenase (LDH) cytotoxicity assessment. In cells exposed to nano-TiO2, lycopene pretreatment significantly boosted the activity of antioxidative enzymes and reduced oxidative stress. Furthermore, when SH-SY5Y cells were subjected to nano-TiO2, lycopene pretreatment stopped neuroinflammation and apoptosis. The findings of this study suggest that lycopene may be an effective neuroprotective against oxidative stress and neuroinflammation and may be used to stop neuronal death or injury in a variety of neurological illnesses.