使用Ocimum Sanctum叶提取物绿色合成银和铜纳米颗粒及其复合材料，显示出增强的抗菌，抗氧化剂和抗癌电位
Green synthesis of silver and copper nanoparticles and their composites using Ocimum sanctum leaf extract displayed enhanced antibacterial, antioxidant and anticancer potentials
影响因子:4.50000
分区:生物学2区 / 生物工程与应用微生物2区 工程：生物医学3区 材料科学：生物材料3区
发表日期:2024 Dec
作者:
M Ashokkumar, K Palanisamy, A Ganesh Kumar, C Muthusamy, K J Senthil Kumar
摘要
绿色合成的银和铜纳米颗粒（NP）以及它们的复合材料表现出各种生物学活性。传统上用作南亚医学的Ocimum Sanctum（Holy Basil）治疗呼吸系统疾病，消化问题，皮肤疾病和炎症疾病。现代科学研究支持这些生物活性。但是，没有研究研究其与NP结合的生物活性。在这项研究中，使用AGNO3和CUSO4·5H2O溶液合成银和铜NP，并使用Ocimum Sanctum Leaf提取物进行了检查，并检查了其抗菌，抗氧化剂和抗癌特性。光谱分析，包括傅立叶变换Infra-RED（FTIR），透射电子显微镜（TEM）和X射线衍射（XRD），阐明了绿色合成的纳米颗粒的物理化学特性（OS-AGNPS和OS-CUNPS）（OS-AGAGNPS和OS-CUNPS），揭示了11.7和13.1 nmm的大小。 OS-AGNP：1：2比例的OS-Cunps纳米复合材料表现出一个抑制区域，范围为8至12 mm，针对测试的细菌病原体。此外，NP及其复合材料表现出有效的抗氧化活性，具有显着的2-二苯基-2-丙烯酰羟基（DPPH）清除活性，在复合材料中观察到，比率为2：1和1：2。此外，他们还显示出对人白血病（Jurkat）癌细胞的潜在抗癌活性。尽管NP及其复合材料之间没有观察到抗癌性质的明显差异，但我们的研究突出了它们定义明确的纳米结构和重要的生物学活性，这表明它们是制药行业的治疗剂的潜力。
Abstract
Green-synthesized silver and copper nanoparticles (NPs), along with their composites, exhibit various biological activities. Ocimum sanctum (Holy basil), traditionally used as medicine in South Asia, treats respiratory disorders, digestive issues, skin diseases and inflammatory conditions. Modern scientific studies support these bioactivities; however, no studies have investigated their bioactivity in combination with NPs. In this study, silver and copper NPs were synthesized using AgNO3 and CuSO4·5H2O solutions, respectively, with Ocimum sanctum leaf extract, and their antibacterial, antioxidant and anticancer properties were examined. Spectroscopic analyses, including Fourier transform infra-red (FTIR), transmission electron microscopy (TEM) and X-ray diffraction (XRD), elucidated the physicochemical characteristics of the green-synthesized nanoparticles (Os-AgNPs and Os-CuNPs), revealing sizes of 11.7 and 13.1 nm, respectively. The Os-AgNPs:Os-CuNPs nano-composite with a 1:2 ratio exhibited a zone of inhibition ranging from 8 to 12 mm against tested bacterial pathogens. Additionally, the NPs and their composites demonstrated potent antioxidant activity, with notable 2-diphenyl-2-picrylhydrazyl (DPPH) scavenging activity observed in composites with ratios of 2:1 and 1:2. Furthermore, they displayed potential anticancer activity against human leukaemia (Jurkat) cancer cells. Although no distinct difference in anticancer property was observed among the NPs and their composites, our study highlights their well-defined nanostructure and significant biological activity, suggesting their potential as therapeutic agents in the pharmaceutical industry.