利用Ocimum sanctum叶提取物绿色合成银和铜纳米粒子及其复合材料显示增强的抗菌、抗氧化和抗癌潜能
Green synthesis of silver and copper nanoparticles and their composites using Ocimum sanctum leaf extract displayed enhanced antibacterial, antioxidant and anticancer potentials
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影响因子:4.5
分区:生物学2区 / 生物工程与应用微生物2区 工程：生物医学3区 材料科学：生物材料3区
发表日期:2024 Dec
作者:
M Ashokkumar, K Palanisamy, A Ganesh Kumar, C Muthusamy, K J Senthil Kumar
DOI:
10.1080/21691401.2024.2399938
摘要
绿色合成的银和铜纳米粒子（NPs）及其复合材料表现出多种生物活性。Ocimum sanctum（圣罗勒），在南亚传统用作药物，治疗呼吸系统疾病、消化问题、皮肤病和炎症等。现代科学研究支持这些生物活性，但尚无研究调查其与纳米粒子结合的生物活性。在本研究中，分别使用AgNO3和CuSO4·5H2O溶液，通过Ocimum sanctum叶提取物合成银和铜纳米粒子，并检测其抗菌、抗氧化和抗癌性能。傅里叶变换红外光谱（FTIR）、透射电子显微镜（TEM）和X射线衍射（XRD）分析揭示了绿色合成的纳米粒子（Os-AgNPs和Os-CuNPs）的理化特性，尺寸分别为11.7和13.1纳米。1:2比例的Os-AgNPs：Os-CuNPs纳米复合材料在测试的细菌病原体中显示出8到12毫米的抑菌圈。此外，纳米粒子及其复合材料表现出强大的抗氧化活性，在比例为2:1和1:2的复合物中观察到显著的2,2-二苯基-1-苦味酸（DPPH）清除活性。它们还显示出对人类白血病（Jurkat）细胞的潜在抗癌活性。虽然在抗癌性能方面，纳米粒子与其复合物之间没有明显差异，但我们的研究强调了它们明确的纳米结构和显著的生物活性，表明它们在药物行业中作为治疗剂的潜力。
Abstract
Green-synthesized silver and copper nanoparticles (NPs), along with their composites, exhibit various biological activities. Ocimum sanctum (Holy basil), traditionally used as medicine in South Asia, treats respiratory disorders, digestive issues, skin diseases and inflammatory conditions. Modern scientific studies support these bioactivities; however, no studies have investigated their bioactivity in combination with NPs. In this study, silver and copper NPs were synthesized using AgNO3 and CuSO4·5H2O solutions, respectively, with Ocimum sanctum leaf extract, and their antibacterial, antioxidant and anticancer properties were examined. Spectroscopic analyses, including Fourier transform infra-red (FTIR), transmission electron microscopy (TEM) and X-ray diffraction (XRD), elucidated the physicochemical characteristics of the green-synthesized nanoparticles (Os-AgNPs and Os-CuNPs), revealing sizes of 11.7 and 13.1 nm, respectively. The Os-AgNPs:Os-CuNPs nano-composite with a 1:2 ratio exhibited a zone of inhibition ranging from 8 to 12 mm against tested bacterial pathogens. Additionally, the NPs and their composites demonstrated potent antioxidant activity, with notable 2-diphenyl-2-picrylhydrazyl (DPPH) scavenging activity observed in composites with ratios of 2:1 and 1:2. Furthermore, they displayed potential anticancer activity against human leukaemia (Jurkat) cancer cells. Although no distinct difference in anticancer property was observed among the NPs and their composites, our study highlights their well-defined nanostructure and significant biological activity, suggesting their potential as therapeutic agents in the pharmaceutical industry.