绿色合成的银和铜纳米颗粒（NP）及其复合材料表现出多种生物活性。圣罗勒（圣罗勒）在南亚传统上用作药物，可治疗呼吸系统疾病、消化问题、皮肤病和炎症性疾病。现代科学研究支持这些生物活性；然而，还没有研究调查它们与纳米粒子结合的生物活性。本研究分别使用 AgNO3 和 CuSO4·5H2O 溶液以及罗勒叶提取物合成了银和铜纳米粒子，并检测了它们的抗菌、抗氧化和抗癌特性。光谱分析，包括傅里叶变换红外 (FTIR)、透射电子显微镜 (TEM) 和 X 射线衍射 (XRD)，阐明了绿色合成纳米粒子（Os-AgNP 和 Os-CuNP）的物理化学特性，揭示了尺寸分别为 11.7 和 13.1nm。比例为 1:2 的 Os-AgNPs:Os-CuNPs 纳米复合材料对测试的细菌病原体表现出 8 至 12mm 的抑制区域。此外，纳米粒子及其复合材料表现出有效的抗氧化活性，在比例为 2:1 和 1:2 的复合材料中观察到显着的 2-二苯基-2-三硝基苯肼 (DPPH) 清除活性。此外，它们还表现出针对人类白血病（Jurkat）癌细胞的潜在抗癌活性。尽管纳米粒子及其复合材料的抗癌特性没有观察到明显差异，但我们的研究强调了它们明确的纳米结构和显着的生物活性，表明它们在制药行业作为治疗剂的潜力。

Green-synthesized silver and copper nanoparticles (NPs), along with their composites, exhibit various biological activities. Ocimum sanctum (Holy basil), traditionally used as medicine in South Asia, treats respiratory disorders, digestive issues, skin diseases and inflammatory conditions. Modern scientific studies support these bioactivities; however, no studies have investigated their bioactivity in combination with NPs. In this study, silver and copper NPs were synthesized using AgNO3 and CuSO4·5H2O solutions, respectively, with Ocimum sanctum leaf extract, and their antibacterial, antioxidant and anticancer properties were examined. Spectroscopic analyses, including Fourier transform infra-red (FTIR), transmission electron microscopy (TEM) and X-ray diffraction (XRD), elucidated the physicochemical characteristics of the green-synthesized nanoparticles (Os-AgNPs and Os-CuNPs), revealing sizes of 11.7 and 13.1 nm, respectively. The Os-AgNPs:Os-CuNPs nano-composite with a 1:2 ratio exhibited a zone of inhibition ranging from 8 to 12 mm against tested bacterial pathogens. Additionally, the NPs and their composites demonstrated potent antioxidant activity, with notable 2-diphenyl-2-picrylhydrazyl (DPPH) scavenging activity observed in composites with ratios of 2:1 and 1:2. Furthermore, they displayed potential anticancer activity against human leukaemia (Jurkat) cancer cells. Although no distinct difference in anticancer property was observed among the NPs and their composites, our study highlights their well-defined nanostructure and significant biological activity, suggesting their potential as therapeutic agents in the pharmaceutical industry.