在头部和颈部癌中的PD-L1测定法比较
Comparison of PD-L1 assays in head and neck carcinoma
影响因子:3.00000
分区:医学3区 / 病理学3区
发表日期:2024 Dec
作者:
Ji-Seon Jeong, Uiree Jo, Gyuheon Choi, Halim Song, Kyung-Ja Cho, Joon Seon Song
摘要
程序性细胞死亡配体1（PD-L1）表达是一种预测性生物标志物，用于响应头颈鳞状细胞癌中免疫检查点抑制剂的反应。考虑到PD-L1测试的抗体和平台范围，必须了解不同染色和评分方法的性能。使用106个组织微阵列（TMA）核心评估ASAN医疗中心的156头和颈粘膜鳞状细胞癌（HNMSCC）病例中的PD-L1表达。评估了三个标准化的PD-L1测定（22C3 PharmDX，SP263和28-8 PharmDX）和一个实验室开发测试（22C3 LDT）：合计的正分数（CPS）≥1，≥20，≥50级和≥50次，tumor阳性得分（TPS），以及TUMOR阳性分数（TPS），具有≥1％，≥20％，≥20％，≥20％，≥20％，〜50％，〜50％。对于CPS（类内相关系数（ICC）范围0.73-0.94）和TPS（ICC范围0.70-0.94）以及TMA和整个幻灯片队列的CPS（ICC）范围0.73-0.94]以及TMA和整个幻灯片队列。通过GWET的AC1分析，可变截止的分层在大多数测定中表明大多数测定中的中等至良好的一致性。使用22C3 PharmDX分析（CPS，P = 0.014； TPS，P = 0.033），PD-L1表达与肿瘤位置显着相关。在PD-L1分析中发现了显着的一致性，这表明它们在HNMSCC中的潜在互换性。
Abstract
Programmed cell death-ligand 1 (PD-L1) expression is a predictive biomarker for response to immune checkpoint inhibitor in head and neck squamous cell carcinoma. Given the range of antibodies and platforms for PD-L1 testing, it is essential to understand the performance of different staining and scoring methods. PD-L1 expression in 156 head and neck mucosal squamous cell carcinoma (HNmSCC) cases at Asan Medical Center was assessed using 106 tissue microarray (TMA) cores and 50 whole slides. Three standardised PD-L1 assays (22C3 pharmDx, SP263, and 28-8 pharmDx) and one laboratory-developed test (22C3 LDT) were evaluated: the combined positive score (CPS) with ≥1, ≥20, and ≥50 cut-offs, and the tumour positive score (TPS) with ≥1%, ≥20%, ≥50% cut-offs. Concordance on a continuous scale among the assays was good to excellent for CPS [intraclass correlation coefficient (ICC) range 0.73-0.94] and TPS (ICC range 0.70-0.94) and in both TMA and whole slides cohorts. Stratification by variable cut-offs demonstrated moderate to good agreement among most assays, as analysed by Gwet's AC1. PD-L1 expression was significantly correlated with tumour location using the 22C3 pharmDx assay (CPS, p=0.014; TPS, p=0.033). Notable concordance was found among PD-L1 assays, suggesting their potential interchangeability in HNmSCC.