对徐长卿根的酸水解产物进行化学研究，分离出 11 种未描述的类固醇，即徐徐花素 A-K (1-11) 和 7 种先前描述的类似物 (12-18)。使用综合光谱分析和参考数据阐明了这些化合物的完整结构。从结构上看，化合物1和2代表了在白首植物中发现的雄甾烷型甾体的第一个例子，化合物3-6和12被描述为具有罕见的8,14-节段甾体核心的孕烷型甾体。在细胞毒活性测定中，化合物16对MCF-7、HCT-116、HeLa和HepG2癌细胞系表现出最强的细胞毒作用，IC50值为9.98-16.42 μM，进一步研究表明它可以诱导细胞凋亡和细胞凋亡。以剂量依赖性方式对 HepG2 细胞进行 G0/G1 期周期停滞。版权所有 © 2024 Elsevier Ltd. 保留所有权利。

Chemical investigation of the acid hydrolysate of Cynanchum bungei roots led to the isolation of eleven undescribed steroids, namely cynbungenins A-K (1-11), and seven previously described analogues (12-18). The complete structures of these compounds were elucidated using the comprehensive spectroscopic analyses and reference data. Structurally, compounds 1 and 2 represent the first example of androstane-type steroids found in the Cynanchum plants, and compounds 3-6 and 12 are characterized as pregnane-type steroids with a rare 8,14-seco-steroid core. In the cytotoxic activity assay, compound 16 displayed the strongest cytotoxic effect against MCF-7, HCT-116, HeLa, and HepG2 cancer cell lines, with IC50 values of 9.98-16.42 μM, and further research indicated that it induced both apoptosis and cell cycle arrest in the G0/G1 phase in a dose-dependent manner toward HepG2 cells.Copyright © 2024 Elsevier Ltd. All rights reserved.