类器官是自组织 3D 细胞培养模型，对于研究多个物种（尤其是人类）的发育和疾病机制非常有价值。这些 3D 工程组织可以在体外模拟人体器官的结构和功能。生成类器官的方法已得到显着改进，以各种方式更好地类似于它们的体内对应物。当前类器官模型的主要限制之一是缺乏功能性血管室。在这里，我们讨论在类器官系统中生成可灌注血管网络的方法。包含灌注血管区室可显着增强类器官系统的生理相关性，并且是建立用于发育、临床和药物开发环境的下一代、更复杂的体外系统的关键一步。版权所有 © 2024 作者。由 Elsevier B.V. 出版。保留所有权利。

Organoids are self-organizing 3D cell culture models that are valuable for studying the mechanisms underlying both development and disease in multiple species, particularly, in humans. These 3D engineered tissues can mimic the structure and function of human organs in vitro. Methods to generate organoids have substantially improved to better resemble, in various ways, their in vivo counterpart. One of the major limitations in current organoid models is the lack of a functional vascular compartment. Here we discuss methodological approaches to generating perfusable blood vessel networks in organoid systems. Inclusion of perfused vascular compartments markedly enhances the physiological relevance of organoid systems and is a critical step in the establishment of next generation, higher-complexity in vitro systems for use in developmental, clinical, and drug-development settings.Copyright © 2024 The Authors. Published by Elsevier B.V. All rights reserved.