冷大气等离子体（CAP）是一种治疗癌症的新方法。它可用于处理液体 - 等离子体激活介质 (PAM) - 然后将其作为活性氧和氮物种 (RONS) 的外源源转移到目标。本研究旨在对不同的 PAM 进行化学表征，并评估其对头颈癌细胞 (HNC) 的体外选择性。PAM 是通过将 2 和 5 mL 细胞培养基在 6 ℃下暴露于 CAP 5、10 和 20 分钟来获得的。毫米工作距离。通过离子色谱法评估阴离子动力学。通过 MTS 和流式细胞术评估人表皮角质形成细胞 (HaCaT) 和 HNC 细胞系 HSC3、HSC4 和 A253 的细胞增殖抑制、细胞凋亡发生和细胞周期修饰。2mL 条件显示细胞增殖显着减少，而 2mL 条件显示细胞增殖显着降低。 5mL效果较温和，但时间依赖性较明显。 HaCaT 不受 5 mL PAM 影响，表明对癌细胞具有选择性。经 CAP 暴露修改的培养基化学成分通过调节细胞周期和诱导癌细胞凋亡来影响细胞增殖，而不影响正常细胞。© 2024 作者。约翰·威利出版的《口腔疾病》

Cold atmospheric plasma (CAP) is a novel approach for cancer treatment. It can be used to treat liquids-plasma-activated media (PAM)-which are then transferred to the target as an exogenous source of reactive oxygen and nitrogen species (RONS). The present study aimed at chemically characterizing different PAM and assessing their in vitro selectivity against head and neck cancer cells (HNC).PAM were obtained by exposing 2 and 5 mL of cell culture medium to CAP for 5, 10 and 20 min at a 6 mm working distance. Anions kinetics was evaluated by ion chromatography. Cell proliferation inhibition, apoptosis occurrence, and cell cycle modifications were assessed by MTS and flow cytometry, on human epidermal keratinocyte (HaCaT) and HNC cell lines HSC3, HSC4 and A253.The 2 mL conditions showed a significant reduction in cell proliferation whereas for the 5 mL the effect was milder, but the time-dependence was more evident. HaCaT were unaffected by the 5 mL PAM, indicating a selectivity for cancer cells.The media chemical composition modified by CAP exposure influenced cell proliferation by modulating cell cycle and inducing apoptosis in cancer cells, without affecting normal cells.© 2024 The Author(s). Oral Diseases published by John Wiley & Sons Ltd.