增加细胞内 dNTP 水平可提高 Prime 编辑效率。
Increasing intracellular dNTP levels improves prime editing efficiency.
发表日期：2024 Sep 25
作者：
Pengpeng Liu, Karthikeyan Ponnienselvan, Thomas Nyalile, Sarah Oikemus, Anya T Joynt, Sukanya Iyer, Karen Kelly, Dongsheng Guo, Pyae P Kyawe, Emma Vanderleeden, Sambra D Redick, Lei Huang, Zexiang Chen, Jeong Min Lee, Celia A Schiffer, David M Harlan, Jennifer P Wang, Charles P Emerson, Nathan D Lawson, Jonathan K Watts, Erik J Sontheimer, Jeremy Luban, Scot A Wolfe
来源：
NATURE BIOTECHNOLOGY
摘要：
在原代细胞类型中，细胞内脱氧核苷酸三磷酸 (dNTP) 水平以细胞周期依赖性方式受到严格调节。我们报告说，通过改善莫洛尼鼠白血病病毒逆转录酶酶特性的突变和增加细胞内 dNTP 水平的治疗，可以提高引物编辑效率。综合起来，这些修改可大幅提高精确编辑率。© 2024。作者获得 Springer Nature America, Inc. 的独家许可。
In primary cell types, intracellular deoxynucleotide triphosphate (dNTP) levels are tightly regulated in a cell cycle-dependent manner. We report that prime editing efficiency is increased by mutations that improve the enzymatic properties of Moloney murine leukemia virus reverse transcriptase and treatments that increase intracellular dNTP levels. In combination, these modifications produce substantial increases in precise editing rates.© 2024. The Author(s), under exclusive licence to Springer Nature America, Inc.