使用光热效应和Au@cu/cu2o-rgo的光热效应和过氧化物酶样活性对α-富蛋白的双模式检测
Dual-mode detection of α-fetoprotein using the photothermal effect and peroxidase-like activity of Au@Cu/Cu2O-rGO
影响因子:4.50000
分区:化学2区 / 生物学2区 生物物理2区 电化学2区 生化与分子生物学3区
发表日期:2025 Feb
作者:
Min Wang, Mingzhe Jiang, Wenjing Lai, Zihan Yan, Tingting Wang, Yu Qi, Chenglin Hong
摘要
α-毒素（AFP）被广泛认为是监测肝细胞癌（HCC）的重要标记，并且使用两种不同的转导机制进行监测是避免造成虚假阳性或假阴性风险的有效方法。在本文中，Au@cu/cu2o-rgo被用作光热转换器以及执行器，以促进过氧化氢的分解（H2O2），该分解进一步设计为用于定量评估AFP的双模式检测的探测器。相对于单个材料，复合纳米材料具有高达54.9％的光热转化效率（η）高达54.9％，催化产生的信号高达1.6倍。基于生成的温度和电流信号，AFP在0.01-100 ng/mL的范围内被敏感检测，检测限（LOD）分别为5.62 pg/ml和1.23 pg/ml。双模式测定法将可移植性与检测人类卫生系统的高精度相结合。
Abstract
α-Fetoprotein (AFP) is widely recognized as an important marker for monitoring hepatocellular carcinoma (HCC), and its monitoring using two different transduction mechanisms is an effective way to avoid the risk of false positives or false negatives. In this paper, Au@Cu/Cu2O-rGO was used as a photothermal converter as well as an actuator to promote the decomposition of hydrogen peroxide (H2O2), which was further designed as a probe for dual-mode detection to quantitatively assess AFP. The composite nanomaterials possessed photothermal conversion efficiencies (η) of up to 54.9 % and catalytically generated signals up to 1.6 times greater, relative to a single material. Based on the generated temperature and current signals, AFP has been sensitively detected in the range of 0.01-100 ng/mL, with limits of detection (LOD) of 5.62 pg/mL and 1.23 pg/mL, respectively. The dual-mode assay combines portability with high accuracy for the detection of human health systems.