利用光热效应和Au@Cu/Cu2O-rGO的过氧化物酶样活性进行α-胎蛋白的双模检测

Dual-mode detection of α-fetoprotein using the photothermal effect and peroxidase-like activity of Au@Cu/Cu2O-rGO

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BIOELECTROCHEMISTRY

影响因子:4.5

分区:化学2区 / 生物学2区生物物理2区电化学2区生化与分子生物学3区

发表日期:2025 Feb

作者: Min Wang, Mingzhe Jiang, Wenjing Lai, Zihan Yan, Tingting Wang, Yu Qi, Chenglin Hong

DOI: 10.1016/j.bioelechem.2024.108822

keywords: Biosensor; Dual-mode immunoassay; Electrochemical analysis; Nanomaterials; Photothermal effect

摘要

α-胎蛋白（AFP）被广泛认为是监测肝细胞癌（HCC）的重要标志物，采用两种不同的转导机制进行监测是一种有效的方法，以避免假阳性或假阴性的风险。本文中，Au@Cu/Cu2O-rGO被用作光热转换器和驱动剂，以促进过氧化氢（H2O2）的分解，进而设计为一种双模检测探针，用于定量评估AFP。这些复合纳米材料的光热转换效率（η）高达54.9%，催化产生的信号相较单一材料提高了1.6倍。基于产生的温度和电流信号，能够在0.01-100 ng/mL范围内灵敏检测AFP，其检测限（LOD）分别为5.62 pg/mL和1.23 pg/mL。这种双模检测方法结合了便携性和高精确度，有助于人体健康系统的检测。

Abstract

α-Fetoprotein (AFP) is widely recognized as an important marker for monitoring hepatocellular carcinoma (HCC), and its monitoring using two different transduction mechanisms is an effective way to avoid the risk of false positives or false negatives. In this paper, Au@Cu/Cu2O-rGO was used as a photothermal converter as well as an actuator to promote the decomposition of hydrogen peroxide (H2O2), which was further designed as a probe for dual-mode detection to quantitatively assess AFP. The composite nanomaterials possessed photothermal conversion efficiencies (η) of up to 54.9 % and catalytically generated signals up to 1.6 times greater, relative to a single material. Based on the generated temperature and current signals, AFP has been sensitively detected in the range of 0.01-100 ng/mL, with limits of detection (LOD) of 5.62 pg/mL and 1.23 pg/mL, respectively. The dual-mode assay combines portability with high accuracy for the detection of human health systems.