氧化应激在健康和体内平衡中的作用引起了科学界的兴趣，因为它与心血管和神经退行性疾病、癌症和其他疾病有关。因此，广泛的研究旨在寻找新的外源性抗氧化剂化合物进行补充。多糖因其抗氧化特性而闻名。然而，通常需要对多糖进行化学修饰来增强这些特性。因此，右旋糖酐与没食子酸 (Dex-Gal) 缀合，然后与岩藻依聚糖 A (FucA) 结合配制混合物，旨在实现比单个多糖更优异的抗氧化活性。采用析因设计将 FucA 和 Dex-Gal 以不同比例组合，产生五种混合物（BLD1、BLD2、BLD3、BLD4 和 BLD5）。对体外抗氧化测试的表面图进行分析，包括总抗氧化能力 (TAC)、还原能力和羟自由基清除能力，指导选择 BLD4 作为最佳配方。在过氧化氢诱导的各种氧化应激条件下对 3T3 成纤维细胞进行的测试表明，与其分离的成分相比，BLD4 提供了增强的保护。由 Dex-Gal 和 FucA 组合而成的 BLD4 配方显示出作为抗氧化策略的前景，其性能优于其单个成分，并表明其作为补充剂在不利健康条件下减轻氧化应激的潜力。

The role of oxidative stress in health and homeostasis has generated interest in the scientific community due to its association with cardiovascular and neurodegenerative diseases, cancer, and other diseases. Therefore, extensive research seeks to identify new exogenous antioxidant compounds for supplementation. Polysaccharides are recognized for their antioxidant properties. However, polysaccharide chemical modifications are often necessary to enhance these properties. Therefore, dextran was conjugated with gallic acid (Dex-Gal) and later combined with fucoidan A (FucA) to formulate blends aimed at achieving superior antioxidant activity compared to individual polysaccharides. A factorial design was employed to combine FucA and Dex-Gal in different proportions, resulting in five blends (BLD1, BLD2, BLD3, BLD4, and BLD5). An analysis of surface graphs from in vitro antioxidant tests, including total antioxidant capacity (TAC), reducing power, and hydroxyl radical scavenging, guided the selection of BLD4 as the optimal formulation. Tests on 3T3 fibroblasts under various conditions of oxidative stress induced by hydrogen peroxide revealed that BLD4 provided enhanced protection compared to its isolated components. The BLD4 formulation, resulting from the combination of Dex-Gal and FucA, showed promise as an antioxidant strategy, outperforming its individual components and suggesting its potential as a supplement to mitigate oxidative stress in adverse health conditions.