基于铈金属有机框架、血红素和氧化石墨烯复合材料的非酶促过氧化氢生物传感器
A non-enzymatic hydrogen peroxide biosensor based on cerium metal-organic frameworks, hemin, and graphene oxide composite
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影响因子:4.5
分区:化学2区 / 生物学2区 生物物理2区 电化学2区 生化与分子生物学3区
发表日期:2025 Feb
作者:
Hamidreza Ghaedamini, Dong-Shik Kim
DOI:
10.1016/j.bioelechem.2024.108823
摘要
本研究开发了一种新型非酶促电化学生物传感器，用于实时检测过氧化氢（H2O2），基于铈金属有机框架（Ce-MOFs）、血红素和氧化石墨烯（GO）复合材料。Ce-MOFs作为血红素包埋的高效载体，而GO增强了复合材料的导电性。扫描电子显微镜（SEM）、X射线衍射（XRD）、傅里叶变换红外（FTIR）光谱、紫外-可见（UV-vis）光谱以及热重分析（TGA）等表征技术确认血红素成功嵌入Ce-MOFs中。Ce-MOFs@血红素/GO修饰的传感器表现出灵敏的H2O2检测能力，归功于Ce-MOFs@血红素的优异电催化活性和GO的高导电性。该传感器在0.05到10 mM的H2O2浓度范围内呈线性响应，检测极限（LOD）为9.3 μM。示范该传感器检测人前列腺癌细胞释放的H2O2，彰显其在复杂生物环境中实时监测细胞氧化应激的潜力。为了进一步评估其实用性，在人血清样品中进行测试，回收率在94.50%到103.29%之间，结果令人满意。此外，得益于复合材料出色的过氧化物酶样活性，传感器对常见干扰物表现出优异的选择性。由Elsevier B.V.发表。
Abstract
This study presents the development of a novel non-enzymatic electrochemical biosensor for the real-time detection of hydrogen peroxide (H2O2) based on a composite of cerium metal-organic frameworks (Ce-MOFs), hemin, and graphene oxide (GO). The Ce-MOFs served as an efficient matrix for hemin encapsulation, while GO enhanced the conductivity of the composite. Characterization techniques including scanning electron microscopy (SEM), X-ray diffraction (XRD), Fourier-transform infrared (FTIR) spectroscopy, UV-vis spectroscopy, and thermogravimetric analysis (TGA) confirmed the successful integration of hemin into the Ce-MOFs. The Ce-MOFs@hemin/GO-modified sensor demonstrated sensitive H2O2 detection due to the exceptional electrocatalytic activity of Ce-MOFs@hemin and the high conductivity of GO. This biosensor exhibited a linear response to H2O2 concentrations from 0.05 to 10 mM with a limit of detection (LOD) of 9.3 μM. The capability of the biosensor to detect H2O2 released from human prostate carcinoma cells was demonstrated, highlighting its potential for real-time monitoring of cellular oxidative stress in complex biological environments. To further assess its practical applicability, the sensor was tested in human serum samples, yielding promising results with recovery values ranging from 94.50 % to 103.29 %. In addition, the sensor showed excellent selectivity against common interfering compounds due to the outstanding peroxidase-like activity of the composite.Published by Elsevier B.V.