本研究开发了一种新型非酶电化学生物传感器，用于实时检测过氧化氢 (H2O2)，其基于铈金属有机框架 (Ce-MOF)、氯化血红素和氧化石墨烯 (GO) 的复合材料。 Ce-MOF 作为氯化血红素封装的有效基质，而 GO 则增强了复合材料的电导率。包括扫描电子显微镜 (SEM)、X 射线衍射 (XRD)、傅里叶变换红外 (FTIR) 光谱、紫外-可见光谱和热重分析 (TGA) 在内的表征技术证实了氯化血红素成功整合到 Ce-MOF 中。由于 Ce-MOFs@hemin 优异的电催化活性和 GO 的高电导率，Ce-MOFs@hemin/GO 修饰的传感器表现出灵敏的 H2O2 检测。该生物传感器对 0.05 至 10 mM 的 H2O2 浓度表现出线性响应，检测限 (LOD) 为 9.3 μM。该生物传感器能够检测人类前列腺癌细胞释放的H2O2，突显了其在复杂生物环境中实时监测细胞氧化应激的潜力。为了进一步评估其实际适用性，该传感器在人体血清样本中进行了测试，取得了令人鼓舞的结果，回收率范围为 94.50% 至 103.29%。此外，由于复合材料具有出色的类过氧化物酶活性，该传感器对常见的干扰化合物表现出优异的选择性。由Elsevier B.V.出版。

This study presents the development of a novel non-enzymatic electrochemical biosensor for the real-time detection of hydrogen peroxide (H2O2) based on a composite of cerium metal-organic frameworks (Ce-MOFs), hemin, and graphene oxide (GO). The Ce-MOFs served as an efficient matrix for hemin encapsulation, while GO enhanced the conductivity of the composite. Characterization techniques including scanning electron microscopy (SEM), X-ray diffraction (XRD), Fourier-transform infrared (FTIR) spectroscopy, UV-vis spectroscopy, and thermogravimetric analysis (TGA) confirmed the successful integration of hemin into the Ce-MOFs. The Ce-MOFs@hemin/GO-modified sensor demonstrated sensitive H2O2 detection due to the exceptional electrocatalytic activity of Ce-MOFs@hemin and the high conductivity of GO. This biosensor exhibited a linear response to H2O2 concentrations from 0.05 to 10 mM with a limit of detection (LOD) of 9.3 μM. The capability of the biosensor to detect H2O2 released from human prostate carcinoma cells was demonstrated, highlighting its potential for real-time monitoring of cellular oxidative stress in complex biological environments. To further assess its practical applicability, the sensor was tested in human serum samples, yielding promising results with recovery values ranging from 94.50 % to 103.29 %. In addition, the sensor showed excellent selectivity against common interfering compounds due to the outstanding peroxidase-like activity of the composite.Published by Elsevier B.V.