基于金属有机框架，Hemin和氧化石墨烯复合材料的非酶过氧化氢生物传感器
A non-enzymatic hydrogen peroxide biosensor based on cerium metal-organic frameworks, hemin, and graphene oxide composite
影响因子:4.50000
分区:化学2区 / 生物学2区 生物物理2区 电化学2区 生化与分子生物学3区
发表日期:2025 Feb
作者:
Hamidreza Ghaedamini, Dong-Shik Kim
摘要
这项研究介绍了一种新型的非酶电化化学生物传感器的发展，用于实时检测过氧化氢（H2O2），基于铜质金属有机框架（CE-MOF），HEMIN和氧化石墨烯（GO）的复合材料。 CE-MOF用作Hemin封装的有效基质，而GO则增强了复合材料的电导率。包括扫描电子显微镜（SEM），X射线衍射（XRD），傅立叶转换红外（FTIR）光谱，UV-VIS光谱和热重测分析（TGA）的表征技术证实了Hemin成功整合到CE-MOFS中。 CE-MOF@hemin/GO改装传感器由于CE-MOFS@hemin的特殊电催化活性和GO的高电导率而显示出敏感的H2O2检测。该生物传感器对从0.05至10 mm的H2O2浓度表现出线性响应，检测极限（LOD）为9.3μm。证明了生物传感器检测从人前列腺癌细胞释放的H2O2的能力，突出了其在复杂的生物学环境中实时监测细胞氧化应激的潜力。为了进一步评估其实际适用性，在人血清样品中测试了该传感器，从而产生了有希望的结果，恢复值范围从94.50％到103.29％。此外，由于复合材料的出色过氧化物酶样活性，传感器对常见干扰化合物的选择性具有出色的选择性。
Abstract
This study presents the development of a novel non-enzymatic electrochemical biosensor for the real-time detection of hydrogen peroxide (H2O2) based on a composite of cerium metal-organic frameworks (Ce-MOFs), hemin, and graphene oxide (GO). The Ce-MOFs served as an efficient matrix for hemin encapsulation, while GO enhanced the conductivity of the composite. Characterization techniques including scanning electron microscopy (SEM), X-ray diffraction (XRD), Fourier-transform infrared (FTIR) spectroscopy, UV-vis spectroscopy, and thermogravimetric analysis (TGA) confirmed the successful integration of hemin into the Ce-MOFs. The Ce-MOFs@hemin/GO-modified sensor demonstrated sensitive H2O2 detection due to the exceptional electrocatalytic activity of Ce-MOFs@hemin and the high conductivity of GO. This biosensor exhibited a linear response to H2O2 concentrations from 0.05 to 10 mM with a limit of detection (LOD) of 9.3 μM. The capability of the biosensor to detect H2O2 released from human prostate carcinoma cells was demonstrated, highlighting its potential for real-time monitoring of cellular oxidative stress in complex biological environments. To further assess its practical applicability, the sensor was tested in human serum samples, yielding promising results with recovery values ranging from 94.50 % to 103.29 %. In addition, the sensor showed excellent selectivity against common interfering compounds due to the outstanding peroxidase-like activity of the composite.Published by Elsevier B.V.