报道了配体框架中含有取代的 2-(2-吡啶基)苯并噻唑 (PyBTh) 基团的三种配合物的合成和光谱表征以及比较生物活性。配体的 6 位已被甲氧基 (Py(OMe)BTh) 或甲基 (Py(Me)BTh) 取代。 Py(OMe)BTh 与 CuCl2 或 Cu(NO3)2·2.5 H2O 反应生成单体 [Cu(Py(OMe)BTh))2(NO3)]NO3·1.5 MeOH、(1·1.5 MeOH) 络合物或分别为二聚体 [Cu(Py(OMe)BTh)Cl2]2 (2)，复合物的核取决于起始 Cu(II) 盐。甲基取代的配体与Cu(NO3)2·2.5 H2O之间的反应导致Cu(Py(Me)BTh)(NO3)2·0.5 THF (3·0.5 THF)的分离。配合物 1-3 已得到充分表征。对所有三种配合物以及[Cu(PyBTh)2(H2O)](BF4)2 (4)（我们之前报道的一种含有未取代的2-(2-吡啶基)苯并噻唑配体的化合物）进行了循环伏安法测量。研究了生物活性，包括浓度依赖性 DNA 结合和切割、抗菌活性和癌细胞毒性。所有复合物均表现出 DNA 切割活性，但发现 2 和 4 的活性最强。机理研究表明，核酸酶活性依赖于主要依赖于 O2- 的氧化机制。抗菌研究表明复合物 4 比 1-3 更有效。使用 1-4、Cu(QBTh)(NO3)2(H2O) 和 Cu(PyBIm)3(BF4)2 对 HeLa、PC-3 和 MCF7 细胞进行癌细胞毒性研究。观察到的毒性差异表明配体及其取代基在调节细胞死亡中的重要性。版权所有 © 2024 Elsevier Inc. 保留所有权利。

The synthesis and spectroscopic characterization of three complexes containing a substituted 2-(2-pyridyl)benzothiazole (PyBTh) group in the ligand frame are reported along with the comparative biological activity. The ligands have been substituted at the 6-position with either a methoxy (Py(OMe)BTh) or a methyl group (Py(Me)BTh). Reaction of Py(OMe)BTh with either CuCl2 or Cu(NO3)2·2.5 H2O yielded the monomeric [Cu(Py(OMe)BTh))2(NO3)]NO3·1.5 MeOH, (1·1.5 MeOH) complex or the dimeric [Cu(Py(OMe)BTh)Cl2]2 (2), respectively, with the nuclearity of the complex dependent on the starting Cu(II) salt. Reaction between the methyl substituted ligand and Cu(NO3)2·2.5 H2O resulted in the isolation of Cu(Py(Me)BTh)(NO3)2·0.5 THF (3·0.5 THF). Complexes 1-3 were fully characterized. Cyclic voltammetry measurements were performed on all three complexes as well as on [Cu(PyBTh)2(H2O)](BF4)2 (4), a compound previously reported by us which contains the unsubstituted 2-(2-pyridyl)benzothiazole ligand. The biological activity was studied and included concentration dependent DNA binding and cleavage, antibacterial activity, and cancer cell toxicity. All complexes exhibited DNA cleavage activity, however 2 and 4 were found to be the most potent. Mechanistic studies revealed that the nuclease activity is dependent on an oxidative mechanism reliant principally on O2-. Antibacterial studies revealed complex 4 was more potent compared to 1-3. Cancer cell toxicity studies were carried out on HeLa, PC-3, and MCF7 cells with 1-4, Cu(QBTh)(NO3)2(H2O) and Cu(PyBIm)3(BF4)2. The differences in the observed toxicities suggests the importance of the ligand and its substituents in modulating cell death.Copyright © 2024 Elsevier Inc. All rights reserved.