我们之前在各种癌症的临床样本和正常培养条件下的 HeLa 细胞中报道了自发的正向-反向突变。为了研究化学刺激对此类突变周期的影响，本研究检查了暴露于含汉黄芩素或酸性培养基的 HeLa 和 A549 细胞中的单核苷酸变异 (SNV) 和拷贝数变异 (CNV)。在汉黄芩素中，两种细胞系均在第 16-18 天期间显示出突变周期。在酸性介质中，两种细胞系均表现出不同程度的多个突变周期。基因组特征共定位分析表明，CNV 往往发生在扩展且不稳定的区域，以及启动子、组蛋白和非编码转录位点附近。此外，在两种类型的化学处理下，在正向-反向突变循环期间，细胞形态发生了表型变化。总之，汉黄芩素或酸性施加的化学应激在不同程度上促进了 HeLa 和 A549 细胞的循环正向-反向突变。© 2024。作者。

Spontaneous forward-reverse mutations were reported by us earlier in clinical samples from various types of cancers and in HeLa cells under normal culture conditions. To investigate the effects of chemical stimulations on such mutation cycles, the present study examined single nucleotide variations (SNVs) and copy number variations (CNVs) in HeLa and A549 cells exposed to wogonin-containing or acidic medium. In wogonin, both cell lines showed a mutation cycle during days 16-18. In acidic medium, both cell lines displayed multiple mutation cycles of different magnitudes. Genomic feature colocalization analysis suggests that CNVs tend to occur in expanded and unstable regions, and near promoters, histones, and non-coding transcription sites. Moreover, phenotypic variations in cell morphology occurred during the forward-reverse mutation cycles under both types of chemical treatments. In conclusion, chemical stresses imposed by wogonin or acidity promoted cyclic forward-reverse mutations in both HeLa and A549 cells to different extents.© 2024. The Author(s).