背景：Heracleum sphondylium 是一种用于罗马尼亚民族药理学的药用植物，已被证明具有显着的生物活性。对抗菌素耐药性的担忧不断升级，基于药用植物和纳米技术的新型高效抗菌剂受到了特别关注。我们报告了一种新型、简单的植物载体的制备，该载体利用了 H. sphondylium 和银纳米颗粒（HS-Ag 系统）的生物活性特性。方法：通过气相色谱-质谱法和电喷雾电离-四极杆飞行时间质谱法测定H. sphondylium 的低代谢特征。通过 X 射线衍射、傅里叶变换红外光谱、拉曼光谱、动态光散射、扫描电子显微镜和能量色散 X 射线光谱分析了创新植物载体的形态结构特性。使用总酚含量、铁还原抗氧化能力和 2,2-二苯基-1-三硝基苯肼 (DPPH) 体外测定来评估抗氧化活性。采用琼脂扩散法对金黄色葡萄球菌、枯草芽孢杆菌、铜绿假单胞菌、大肠杆菌进行抗菌活性筛选。 3-(4,5-二甲基噻唑-2-基)-2,5-二苯基溴化四唑 (MTT) 测定评估了对正常人真皮成纤维细胞 (NHDF) 和宫颈癌 (HeLa) 细胞的体外潜在细胞毒性。结果：共检出萜类、黄酮类、酚酸类、香豆素类、苯丙素类、环烯醚萜类、氨基酸、植物甾醇、脂肪酸等88种生物分子。与 H. sphondylium 相比，HS-Ag 植物载体增强了抑制所有测试细菌菌株生长的功效，并对 HeLa 细胞活力表现出显着抑制作用。结论：新型 HS-Ag 植物载体系统有望实现广泛的医疗应用。这些数据证实了增强抗菌剂创新领域相关理论理解的能力。

Background: Heracleum sphondylium, a medicinal plant used in Romanian ethnopharmacology, has been proven to have remarkable biological activity. The escalating concerns surrounding antimicrobial resistance led to a special attention being paid to new efficient antimicrobial agents based on medicinal plants and nanotechnology. We report the preparation of a novel, simple phytocarrier that harnesses the bioactive properties of H. sphondylium and silver nanoparticles (HS-Ag system). Methods: H. sphondylium's low metabolic profile was determined through gas chromatography-mass spectrometry and electrospray ionization-quadrupole time-of-flight-mass spectrometry. The morphostructural properties of the innovative phytocarrier were analyzed by X-ray diffraction, Fourier-transform infrared spectroscopy, Raman spectroscopy, dynamic light scattering, scanning electron microscopy, and energy-dispersive X-ray spectroscopy. The antioxidant activity was evaluated using total phenolic content, ferric reducing antioxidant power, and 2,2-diphenyl-1-picrylhydrazyl (DPPH) in vitro assays. The antimicrobial activity screening against Staphylococcus aureus, Bacillus subtilis, Pseudomonas aeruginosa, and Escherichia coli was conducted using the agar well diffusion method. The 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay estimated the in vitro potential cytotoxicity on normal human dermal fibroblasts (NHDF) and cervical cancer (HeLa) cells. Results: A total of 88 biomolecules were detected, such as terpenoids, flavonoids, phenolic acids, coumarins, phenylpropanoids, iridoids, amino acids, phytosterols, fatty acids. The HS-Ag phytocarrier heightened efficacy in suppressing the growth of all tested bacterial strains compared to H. sphondylium and exhibited a significant inhibition of HeLa cell viability. Conclusions: The new HS-Ag phytocarrier system holds promise for a wide range of medical applications. The data confirm the capacity to augment the pertinent theoretical understanding in the innovative field of antimicrobial agents.