卵巢癌（OC）代表高度多样化的肿瘤微环境（TME）的生态系统。肿瘤浸润淋巴细胞（TIL）的存在与增强的免疫反应和长期生存有关。在这篇综述中，我们提出了新的证据，表明细胞串扰严格调节 TME 内 TIL 的分布，强调需要更好地了解促进或阻碍 OC 中 T 细胞浸润的关键细胞网络。我们还捕获了能够解析细胞间串扰的新兴方法和计算技术。最后，我们提出了创新的离体 TME 模型，可用于绘制和扰乱细胞通信，以增强 T 细胞浸润和免疫反应性。版权所有 © 2024 作者。由爱思唯尔公司出版。保留所有权利。

Ovarian cancer (OC) represents ecosystems of highly diverse tumor microenvironments (TMEs). The presence of tumor-infiltrating lymphocytes (TILs) is linked to enhanced immune responses and long-term survival. In this review we present emerging evidence suggesting that cellular crosstalk tightly regulates the distribution of TILs within the TME, underscoring the need to better understand key cellular networks that promote or impede T cell infiltration in OC. We also capture the emergent methodologies and computational techniques that enable the dissection of cell-cell crosstalk. Finally, we present innovative ex vivo TME models that can be leveraged to map and perturb cellular communications to enhance T cell infiltration and immune reactivity.Copyright © 2024 The Author(s). Published by Elsevier Inc. All rights reserved.