长非编码 RNA (lncRNA) 核副斑组装转录物 1 (NEAT1) 在癌症进展中表现出多样化且复杂的功能。尽管有报道表明其在多种癌症中具有肿瘤抑制和致癌作用，但其在头颈鳞状细胞癌（HNSCC）中的具体作用仍不清楚。本研究旨在探讨 NEAT1 表达水平与 HNSCC 患者生存结果之间的关联。收集了 92 名 HNSCC 患者的肿瘤和非癌性匹配组织 (NCMT) 的配对组织样本。使用 RT-qPCR 分析 NEAT1 表达。对患者的临床特征、接受的治疗和生存率进行评估，以确定与 NEAT1 表达的相关性，并探讨其与酒精、槟榔和吸烟的关系。此外，我们还检测了槟榔碱对正常人口腔角质形成细胞 (NHOK) 和成纤维细胞 (NHOF) 中 NEAT1 表达的影响。该研究表明，与 NCMT 相比，口腔癌组织中 NEAT1 表达显着下调。同时，槟榔碱增加了 NHOK 和 NHOF 细胞中 NEAT1 的表达。然而，NEAT1 表达下调的患者表现出更高的总体生存率，特别是那些未接受化疗或放疗的患者。NEAT1 表达水平与 HNSCC 患者的生存结果相关，表达上调表明预后较差，表明该 lncRNA 可能有助于癌症的侵袭性，尤其是在没有积极治疗的情况下。这些发现表明 NEAT1 可能作为 HNSCC 的潜在预后生物标志物，但需要进一步研究来阐明其在癌症进展中的作用及其作为治疗靶点的潜力。© 2024 中华民国牙科科学协会。 Elsevier B.V. 的出版服务

The long non-coding RNA (lncRNA) nuclear paraspeckle assembly transcript 1 (NEAT1) exhibits diverse and complicated functions in cancer progression. Despite reports suggesting both tumor-suppressive and oncogenic effects in various cancers, its specific role in head and neck squamous cell carcinoma (HNSCC) remains unclear. This study aimed to investigate the association between NEAT1 expression levels and survival outcomes in HNSCC patients.Paired tissue samples of tumor and non-cancerous matching tissues (NCMT) from 92 HNSCC patients were collected. NEAT1 expression was analyzed using RT-qPCR. Clinical characteristics, treatment received, and survival rates of the patients were assessed to determine the correlation with NEAT1 expression and explore its association with alcohol, betel quid, and cigarette use. Additionally, we examined the effect of arecoline on NEAT1 expression in normal human oral keratinocytes (NHOK) and fibroblasts (NHOF).The study revealed a significant downregulation of NEAT1 expression in oral cancer tissues compared to NCMT. Meanwhile, arecoline increased NEAT1 expression in NHOK and NHOF cells. However, patients with downregulated NEAT1 expression exhibited higher overall survival rates, particularly in those who did not receive chemotherapy or radiotherapy.NEAT1 expression levels are associated with survival outcomes in HNSCC patients, with upregulated expression indicating a worse prognosis, suggesting this lncRNA might contribute to cancer aggressiveness, especially in the absence of active treatment. These findings indicate NEAT1 may serve as a potential prognostic biomarker in HNSCC, but further research is required to elucidate its role in cancer progression and its potential as a therapeutic target.© 2024 Association for Dental Sciences of the Republic of China. Publishing services by Elsevier B.V.