手持式非热等离子体（HNP）处理可能会改变聚芳醚酮（PAEK）牙科种植材料的表面特性、骨代谢和炎症反应。本研究测试了 HNP 处理是否可以提高 PAEK 材料的生物相容性、表面亲水性、表面自由能 (SFE) 以及细胞粘附和矿化能力。钛 (Ti)、氧化锆 (Zr)、聚醚醚酮 ( PEEK [PE]）和聚醚酮酮（PEKK [PK]）经过 HNP 处理，分别称为 TiPL、ZrPL、PEPL 和 PKPL。使用测角仪检查水表面反应。在所有样品上培养 MG-63 细胞，以评估细胞活力、细胞毒性、细胞附着和矿化特征。使用酶联免疫吸附剂测量促炎细胞因子（肿瘤坏死因子-α 和白介素-6）和关键矿化标志物（碱性磷酸酶 [ALKP]、骨桥蛋白 [OPN] 和牙本质基质蛋白 1 [DMP1]）的表达与未处理的样品相比，HNP 处理的样品表现出显着增强的表面亲水性和 SFE。所有样品的细胞活力仍然很高，表明没有细胞毒性作用。 HNP 处理显着增强了 MG-63 细胞的粘附和增殖。在血浆处理的 PEPL 和 PKPL 样本中观察到 ALKP 和 OPN 水平升高，而 DMP1 水平仅在 PKPL 样本中显着升高。所有样品的促炎细胞因子水平都很低，表明没有炎症反应。HNP处理的PAEK增强了表面亲水性和SFE，以及优异的细胞粘附和矿化能力，因此可能是良好的临床牙种植材料。© 2024中华民国齿科医学会. Elsevier B.V. 的出版服务

The handheld nonthermal plasma (HNP) treatment may alter the surface properties, bone metabolism, and inflammatory reactions of polyaryletherketone (PAEK) dental implant materials. This study tested whether the HNP treatment might increase the biocompatibility, surface hydrophilicity, surface free energies (SFEs), and the cell adhesion and mineralization capability of PAEK materials.Disk-shaped samples of titanium (Ti), zirconia (Zr), polyetheretherketone (PEEK [PE]), and polyetherketoneketone (PEKK [PK]) were subjected to HNP treatment and termed as TiPL, ZrPL, PEPL, and PKPL, respectively. Water-surface reactions were examined using a goniometer. MG-63 cells were cultured on all samples to assess the cell viability, cytotoxicity, cell attachment, and mineralization characteristics. The expression of pro-inflammatory cytokines (tumor necrosis factor-alpha and interleukin-6) and key mineralization markers (alkaline phosphatase [ALKP], osteopontin [OPN], and dentin matrix protein 1 [DMP1]) was measured using enzyme-linked immunosorbent assay kits.The HNP-treated samples exhibited significantly enhanced surface hydrophilicities and SFEs compared to the untreated samples. The cell viability remained high across all samples, indicating no cytotoxic effects. The HNP treatment significantly enhanced MG-63 cell adherence and proliferation. Elevated levels of ALKP and OPN were observed for the plasma-treated PEPL and PKPL specimens, while DMP1 levels increased significantly only in the PKPL specimen. Pro-inflammatory cytokine levels were low across all samples, suggesting no inflammatory response.The HNP-treated PAEKs have enhanced the surface hydrophilicity and SFEs as well as superior cell adhesion and mineralization capability, and thus may be good clinical dental implant materials.© 2024 Association for Dental Sciences of the Republic of China. Publishing services by Elsevier B.V.