脂质成像质谱法：迈向新的分子组织学
Lipid imaging mass spectrometry: Towards a new molecular histology
影响因子:3.30000
分区:生物学2区 / 生物物理2区 生化与分子生物学3区 细胞生物学3区
发表日期:2025 Jan
作者:
Ibai Calvo, Olatz Fresnedo, Lorena Mosteiro, José I López, Gorka Larrinaga, José A Fernández
摘要
脂质研究吸引了更多的关注，因为这些分子是了解细胞代谢以及基因型和表型之间的联系的关键组成部分。新的和强大的技术的发展也为脂质的研究加剧了，能够在一次运行中识别出越来越多的物种，并且浓度降低。这样的关键发展之一是图像技术，可以使脂质分布在具有细胞分辨率的组织上可视化。得益于此类技术报告的空间信息，在固定的代谢阶段，可以将脂肪组性状与单个细胞联系起来，这极大地促进了与癌症等不同病理状况相关的代谢变化。此外，脂质的形象正在成为一种新的分子组织学，有很大的机会在医院的诊断单位产生影响。在这里，我们检查了技术的当前状态，并分析了将其带入诊断单元的下一步。为了说明这项技术的潜在和挑战，我们提出了一项有关清晰细胞肾细胞癌的案例研究，这是一个很好的模型，用于分析恶性肿瘤，这是由于其明显的细胞和分子异质性。
Abstract
Lipid research is attracting greater attention, as these molecules are key components to understand cell metabolism and the connection between genotype and phenotype. The study of lipids has also been fueled by the development of new and powerful technologies, able to identify an increasing number of species in a single run and at decreasing concentrations. One of such key developments has been the image techniques that enable the visualization of lipid distribution over a tissue with cell resolution. Thanks to the spatial information reported by such techniques, it is possible to associate a lipidome trait to individual cells, in fixed metabolic stages, which greatly facilitates understanding the metabolic changes associated to diverse pathological conditions, such as cancer. Furthermore, the image of lipids is becoming a kind of new molecular histology that has great chances to make an impact in the diagnostic units of the hospitals. Here, we examine the current state of the technology and analyze what the next steps to bring it into the diagnosis units should be. To illustrate the potential and challenges of this technology, we present a case study on clear cell renal cell carcinoma, a good model for analyzing malignant tumors due to their significant cellular and molecular heterogeneity.