基于适体识别释放策略的SERS分析平台，用于有效且敏感的结直肠癌病变
SERS Analysis Platform Based on Aptamer Recognition-Release Strategy for Efficient and Sensitive Diagnosis of Colorectal Precancerous Lesions
影响因子:6.50000
分区:医学2区 / 药学2区 纳米科技3区
发表日期:2024
作者:
Fengsong Chen, Yanhua Huang, Yongxia Liu, Yanwen Zhuang, Xiaowei Cao, Xiaogang Qin
摘要
大肠癌（CRC）已成为全球重大的公共卫生挑战，由于其发病率高和死亡率，要求立即关注。常规的CRC筛查对于早期发现癌前病变和CRC。该平台利用适体识别释放策略来实现蛋白质肿瘤标记的有效和敏感的检测。在检测过程中，适体和靶蛋白之间的强亲和力和高特异性导致竞争替代最初与底物表面结合的SERS纳米探针。结果，携带拉曼记者基因的纳米螺旋射线被移开，导致SERS信号强度的降低。该平台表现出良好的检测性能，在15分钟内快速检测（LOD）限制（LOD）低至6.2×10-12 g/ml，HNRNP A1和6.51×10-12 g/ml fors s11 00p fors s11 00p。使用SERS平台分析的临床样品与酶联免疫吸附测定法（ELISA）结果表现出很高的一致性。该平台为早期检测，风险评估和治疗癌症前癌前病变提供了强有力的支持，具有广泛的临床应用潜力。
Abstract
Colorectal cancer (CRC) has become a significant global public health challenge, demanding immediate attention due to its high incidence and mortality rates. Regular CRC screening is essential for the early detection of precancerous lesions and CRC.: We developed a novel surface-enhanced Raman scattering (SERS) analysis platform that employs high-throughput microarray chips as carriers and Au/SnO2 nanoring arrays (Au/SnO2 NRAs) as substrates. This platform utilizes an aptamer recognition-release strategy to achieve efficient and sensitive detection of protein tumor markers. In the detection process, the strong affinity and high specificity between the aptamer and the target protein result in competitive replacement of the SERS nanoprobes originally bound to the substrate surface. As a result, the SERS nanoprobes carrying Raman reporter genes are dislodged, leading to a reduction in the SERS signal intensity.The platform demonstrated excellent detection performance, with rapid detection completed within 15 minutes and limits of detection (LOD) as low as 6.2×10-12 g/mL for hnRNP A1 and 6.51×10-12 g/mL for S100P. Clinical samples analyzed using the SERS platform showed high consistency with enzyme-linked immunosorbent assay (ELISA) results.This platform offers strong support for the early detection, risk assessment, and treatment monitoring of colorectal cancer precancerous lesions, with broad potential for clinical applications.