新型正电子发射断层扫描 (PET) 成像示踪剂 [18F]F-AraG 以活化的 T 细胞为目标，为提高我们对免疫肿瘤过程的理解提供了一种潜在的方法。本研究的目的是确定最佳药代动力学模型来量化非小细胞肺癌 (NSCLC) 患者肿瘤病变 [18F]F-AraG 的摄取，并根据药代动力学摄取参数验证不同时间间隔的简化测量.10 名早期 NSCLC 患者和 3 名晚期 NSCLC 患者接受了至少 60 分钟的动态 PET 扫描。最多在七个时间点采集静脉和/或动脉血样。使用单组织可逆（1T2k）、两组织不可逆（2T3k）和两组织可逆（2T4k）血浆输入模型分析肿瘤病变时间活动曲线和代谢物校正输入函数。简化的摄取测量，例如标准化摄取值（SUV）和肿瘤与血液（TBR）或肿瘤与血浆比率（TPR），在不同的时间间隔进行评估。全血和血浆放射性浓度显示快速清除[18F]F-AraG。代谢物分析显示代谢率较低，注射后 70 分钟时，血液中发现的总放射性平均有 79% (SD = 9.8%) 对应于完整的 [18F]F-AraG。 2T3k 模型最适合时间活动曲线。 SUV（校正后的体重 (BW)、去脂体重 (LBM) 或体表面积 (BSA)）、TBR 和 TPR 在注射时间 20 至 70 分钟之间的任何时间间隔均呈强正相关。对抗 2T3k 衍生的 Ki。注射时间 60-70 分钟时 TBR 的相关性2T3K 衍生的 Ki (r (df = 20) = 0.87, p < 0.01) 强于 SUVBW (r (df = 20) = 0.80, p < 0.01)。 肿瘤病变 [18F]F-AraG 摄取NSCLC患者的特点是2T3k模型。 TBR 和 TPR 在简化 NSCLC 患者肿瘤病变 [18F]F-AraG 摄取量化方面表现出最大潜力。© 2024。作者。

The novel positron emission tomography (PET) imaging tracer, [18F]F-AraG, targets activated T-cells, offering a potential means to improve our understanding of immune-oncological processes. The aim of this study was to determine the optimal pharmacokinetic model to quantify tumour lesion [18F]F-AraG uptake in patients with non-small cell lung cancer (NSCLC), and to validate simplified measures at different time intervals against the pharmacokinetic uptake parameter.Ten patients with early-stage NSCLC and three patients with advanced NSCLC underwent a dynamic PET scan of minimal 60 min. Venous and/or arterial blood sampling was obtained at maximum seven time points. Tumour lesion time activity curves and metabolite-corrected input functions were analysed using single-tissue reversible (1T2k), two-tissue irreversible (2T3k) and two-tissue reversible (2T4k) plasma input models. Simplified uptake measures, such as standardised uptake value (SUV) and tumour-to-blood (TBR) or tumour-to-plasma ratio (TPR), were evaluated for different time intervals.Whole-blood and plasma radioactivity concentrations showed rapid clearance of [18F]F-AraG. Metabolite analysis revealed a low rate of metabolism, at 70 min p.i., on average, 79% (SD = 9.8%) of the total radioactivity found in blood corresponded to intact [18F]F-AraG. The time activity curves were best fitted by the 2T3k model. Strong positive correlations were found for SUV (body weight (BW), lean body mass (LBM) or body surface area (BSA) corrected), TBR and TPR for any time interval between 20 and 70 min p.i. against the 2T3k-derived Ki. The correlation of TBR at 60-70 min p.i. with 2T3K-derived Ki (r (df = 20) = 0.87, p < 0.01), was stronger than for SUVBW (r (df = 20) = 0.80, p < 0.01).Tumour lesion [18F]F-AraG uptake in patients with NSCLC is characterised by a 2T3k model. TBR and TPR show most potential for simplified quantification of tumour lesion [18F]F-AraG uptake in patients with NSCLC.© 2024. The Author(s).