腺苷到肌苷 (A-to-I) RNA 编辑的失调与癌症进展有关。然而，由于缺乏有效的识别方法，对 A-to-I RNA 编辑如何纳入 miRNA 调控以调节癌症基因表达的全面理解仍不清楚。为此，我们引入了一种名为 REMR 的基于信息论的算法，基于多组学系统地识别 10 种主​​要癌症类型中的 12,006 个 A-to-I RNA 编辑介导的 miRNA 调控三联体（RNA 编辑位点、miRNA 和基因）来自癌症基因组图谱 (TCGA) 的分析数据。通过对功能富集、转录调控网络和蛋白质-蛋白质相互作用（PPI）网络的分析，我们发现RNA编辑介导的miRNA调控可能影响关键的癌症相关功能，例如细胞凋亡、细胞周期、耐药性和免疫。此外，三联体可以作为生物标志物，用于对具有不同预后或药物反应的癌症亚型进行分类，突出了这种调节的临床相关性。此外，还构建了一个在线资源（http://www.jianlab.cn/REMR/）以支持方便地检索我们的研究结果。总之，我们的研究系统地剖析了 RNA 编辑介导的 miRNA 调控，从而为理解 RNA 编辑作为癌症表观转录组调控因子的机制提供了宝贵的资源。© 2024 作者。

Dysregulation of adenosine-to-inosine (A-to-I) RNA editing has been implicated in cancer progression. However, a comprehensive understanding of how A-to-I RNA editing is incorporated into miRNA regulation to modulate gene expression in cancer remains unclear, given the lack of effective identification methods. To this end, we introduced an information theory-based algorithm named REMR to systematically identify 12,006 A-to-I RNA editing-mediated miRNA regulatory triplets (RNA editing sites, miRNAs, and genes) across ten major cancer types based on multi-omics profiling data from The Cancer Genome Atlas (TCGA). Through analyses of functional enrichment, transcriptional regulatory networks, and protein-protein interaction (PPI) networks, we showed that RNA editing-mediated miRNA regulation potentially affects critical cancer-related functions, such as apoptosis, cell cycle, drug resistance, and immunity. Furthermore, triplets can serve as biomarkers for classifying cancer subtypes with distinct prognoses or drug responses, highlighting the clinical relevance of such regulation. In addition, an online resource (http://www.jianglab.cn/REMR/) was constructed to support the convenient retrieval of our findings. In summary, our study systematically dissected the RNA editing-mediated miRNA regulations, thereby providing a valuable resource for understanding the mechanism of RNA editing as an epitranscriptomic regulator in cancer.© 2024 The Authors.