积云细胞中的基因表达水平与牛卵母细胞的发育能力相关
Gene expression levels in cumulus cells are correlated with developmental competence of bovine oocytes
影响因子:2.50000
分区:农林科学2区 / 兽医学1区 生殖生物学3区
发表日期:2025 Jan 01
作者:
Mayuko Anazawa, Shiori Ashibe, Yoshikazu Nagao
摘要
许多胚胎未能发育到胚泡阶段，通过体外培养产生的哺乳动物胚胎受到阻碍。即使在视觉上选择具有良好形态并将其用于培养物的积云 - 素核细胞复合物（COC）也会发生。由于积云细胞对于卵母细胞的成熟和随后的胚胎发育很重要，因此我们在这里比较了COC的库卢斯细胞中在体外发展到胚泡阶段的基因表达模式与未发展的COC的基因表达模式。从选择用于体外培养的牛COC中吸出积云。通过筛选裂解和发育到胚泡阶段来评估卵母细胞发育能力。使用收集到的卵石细胞定量FSH受体（FSHR），胰岛素样生长因子-1受体（IGF-1R），抗肿瘤激素（AMH），AMH受体II（AMHRII）（AMHRII），表皮生长因子受体（EGFR），雌激素受体β（Eruk），/b lymeb（Eruk），/blymemβ（Eruk），/blymemβ（er limk），/b lymβ（egfr），/b lymβ（egfr），/b lymβ（egfr），/b lymβ（egfr），/b lymβ（egfr），/半胱氨酸 - 天冬氨酸蛋白酶-3（caspase-3）。我们发现，与未开发的COC相比，FSHR，IGF-1R，AMH和EGFR的表达水平较高，而COC的COCS中的表达水平较高，而Bax和Caspase-3的表达水平较低，而Bax和Caspase-3的表达水平较低，而COC的COCS的表达水平则较低，而COC的COCS的表达水平较低。在FSHR和IGF-1R表达（r = 0.59）和EGFR表达（r = 0.43）之间发现了正相关的阳性相关性（r = 0.43）。我们的发现表明，积云细胞中的基因表达水平与牛卵母细胞的发育能力相关。因此，云细胞中基因表达的测量提供了预测卵母细胞发育能力的非侵入性手段。
Abstract
The generation of mammalian embryos by in vitro culture is hampered by the failure of many of the embryos to develop to the blastocyst stage. This problem occurs even when cumulus-oocyte complexes (COCs) with good morphology are visually selected and used for culture. Because cumulus cells are important for oocyte maturation and subsequent embryo development, here we compared gene expression patterns in cumulus cells of COCs that developed in vitro to the blastocyst stage with those of COCs that failed to develop. Cumulus cells were aspirated from bovine COCs selected for in vitro culture. Oocyte developmental competence was evaluated by screening for cleavage and development to the blastocyst stage. The collected cumulus cells were used to quantify mRNA levels of FSH receptor (FSHR), insulin-like growth factor-1 receptor (IGF-1R), anti-Müllerian hormone (AMH), AMH receptor II (AMHRII), epidermal growth factor receptor (EGFR), estrogen receptor β (ERβ), B cell lymphoma/leukemia-2 associated X (Bax), and cysteine-aspartic acid protease-3 (Caspase-3). We found that the expression levels of FSHR, IGF-1R, AMH, and EGFR were higher in cumulus cells from COCs that developed to blastocysts as compared with those that failed to develop, whereas expression levels of Bax and Caspase-3 were lower in cumulus cells of COCs that matured to the blastocyst stage. Positive correlations were found between FSHR and IGF-1R expression (r = 0.59) and between ERβ and EGFR expression (r = 0.43) in cumulus cells from COCs that developed to the blastocyst stage. Our findings indicate that gene expression levels in cumulus cells are correlated with the developmental competence of bovine oocytes. Measurement of gene expression in cumulus cells therefore offers a non-invasive means of predicting oocyte developmental competence.