通过体外培养产生哺乳动物胚胎受到许多胚胎未能发育至囊胚阶段的阻碍。即使在视觉上选择具有良好形态的卵丘-卵母细胞复合物（COC）并用于培养时，也会出现此问题。由于卵丘细胞对于卵母细胞成熟和随后的胚胎发育很重要，因此在这里我们比较了体外发育至囊胚阶段的COC与未能发育的COC的卵丘细胞中的基因表达模式。从选择用于体外培养的牛 COC 中吸出卵丘细胞。通过筛选卵母细胞发育和发育至囊胚阶段来评估卵母细胞发育能力。收集的卵丘细胞用于定量FSH受体（FSHR）、胰岛素样生长因子-1受体（IGF-1R）、抗苗勒管激素（AMH）、AMH受体II（AMHRII）、表皮生长因子受体的mRNA水平(EGFR)、雌激素受体 β (ERβ)、B 细胞淋巴瘤/白血病 2 相关 X (Bax) 和半胱氨酸天冬氨酸蛋白酶 3 (Caspase-3)。我们发现，与未发育的COC相比，发育成囊胚的COC卵丘细胞中FSHR、IGF-1R、AMH和EGFR的表达水平较高，而Bax和Caspase-3的表达水平在卵丘中较低成熟至囊胚阶段的 COC 细胞。在发育至囊胚期的 COC 卵丘细胞中，FSHR 和 IGF-1R 表达（r = 0.59）以及 ERβ 和 EGFR 表达（r = 0.43）之间存在正相关。我们的研究结果表明，卵丘细胞中的基因表达水平与牛卵母细胞的发育能力相关。因此，卵丘细胞基因表达的测量提供了一种预测卵母细胞发育能力的非侵入性方法。版权所有 © 2024 作者。由爱思唯尔公司出版。保留所有权利。

The generation of mammalian embryos by in vitro culture is hampered by the failure of many of the embryos to develop to the blastocyst stage. This problem occurs even when cumulus-oocyte complexes (COCs) with good morphology are visually selected and used for culture. Because cumulus cells are important for oocyte maturation and subsequent embryo development, here we compared gene expression patterns in cumulus cells of COCs that developed in vitro to the blastocyst stage with those of COCs that failed to develop. Cumulus cells were aspirated from bovine COCs selected for in vitro culture. Oocyte developmental competence was evaluated by screening for cleavage and development to the blastocyst stage. The collected cumulus cells were used to quantify mRNA levels of FSH receptor (FSHR), insulin-like growth factor-1 receptor (IGF-1R), anti-Müllerian hormone (AMH), AMH receptor II (AMHRII), epidermal growth factor receptor (EGFR), estrogen receptor β (ERβ), B cell lymphoma/leukemia-2 associated X (Bax), and cysteine-aspartic acid protease-3 (Caspase-3). We found that the expression levels of FSHR, IGF-1R, AMH, and EGFR were higher in cumulus cells from COCs that developed to blastocysts as compared with those that failed to develop, whereas expression levels of Bax and Caspase-3 were lower in cumulus cells of COCs that matured to the blastocyst stage. Positive correlations were found between FSHR and IGF-1R expression (r = 0.59) and between ERβ and EGFR expression (r = 0.43) in cumulus cells from COCs that developed to the blastocyst stage. Our findings indicate that gene expression levels in cumulus cells are correlated with the developmental competence of bovine oocytes. Measurement of gene expression in cumulus cells therefore offers a non-invasive means of predicting oocyte developmental competence.Copyright © 2024 The Authors. Published by Elsevier Inc. All rights reserved.