外泌体作为细胞间通讯的载体，可以作为探索癌症早期诊断的有前景的生物标志物。胰腺癌是胰腺常见的恶性肿瘤，起病隐匿，早期诊断困难。本研究的目的是通过磷脂酰丝氨酸分子印迹聚合物（PS-MIP）捕获尿液样本中的外泌体。透射电子显微镜和纳米颗粒跟踪分析以及蛋白质印迹表明我们的分子印迹材料可以有效捕获尿液外泌体。三个平行测试验证了质谱测定的重现性和材料捕获效率的稳定性。质谱与非靶向蛋白质组学相结合，显示 5 名胰腺癌患者和 5 名健康对照者的外泌体中蛋白质表达存在差异。与健康对照相比，胰腺癌患者蛋白质组谱最显着的变化是 SLC9A3R1、SPAG9 和铁蛋白轻链 (FTL) 的过度表达。这些蛋白质可能在诊断和预后评估中发挥重要作用，支持进一步的科学和临床研究关于胰腺癌。

Exosomes, as carriers of cell-to-cell communication, can serve as promising biomarkers for probing the early diagnosis of cancer. Pancreatic cancer is a common malignant tumor of the pancreas with an insidious onset and difficult early diagnosis. The aim of this study was to capture exosomes in urine samples by phosphatidylserine-molecularly imprinted polymers (PS-MIPs). Transmission electron microscopy and nanoparticle tracking analysis as well as Western blot showed that our molecularly imprinted material can effectively capture urinary exosomes. Three parallel tests verified the reproducibility of the mass spectrometry assay and the stability of the material capture efficiency. Mass Spectrometry with nontargeted proteomics was combined to show differentially expressed proteins in exosomes between 5 pancreatic cancer patients and 5 healthy controls. The most significant changes in the proteomic profile in pancreatic cancer patients compared to healthy controls were the overexpression of SLC9A3R1, SPAG9, and ferritin light chain (FTL) These proteins may have an important role in diagnosis and prognostic assessment, supporting further scientific and clinical studies on pancreatic cancer.