细菌表面展示与荧光激活细胞分选相结合是一种多功能且强大的系统，也是用于产生治疗性亲和蛋白的噬菌体展示的有趣替代方法。该系统能够实时监测和分选细胞群，这为药物开发提供了独特的可能性。它已被用于开发几种亲和体分子，目前正在临床前评估其用于癌症和神经退行性疾病等治疗和诊断的效果。此外，它还可以应用于药物设计的其他领域，例如绘制表位和进化酶特异性。© 2024 作者。约翰·威利 (John Wiley) 出版的《微生物生物技术》

Bacterial surface display in combination with fluorescence-activated cell sorting is a versatile and robust system and an interesting alternative approach to phage display for the generation of therapeutic affinity proteins. The system enables real-time monitoring and sorting of cell populations, which presents unique possibilities for drug development. It has been used to develop several affibody molecules currently being evaluated preclinically for the treatment and diagnosis of, for example, cancer and neurodegenerative diseases. Additionally, it can be implemented in other areas of drug design, such as for mapping epitopes and evolving enzyme specificities.© 2024 The Author(s). Microbial Biotechnology published by John Wiley & Sons Ltd.