越来越多的证据表明肠道微生物群在结直肠癌 (CRC) 的发生和进展中发挥着重要作用。特别是，口腔病原体的过多存在与结直肠癌有关。本研究的目的是进一步调查 CRC 患者的粪便微生物状况，重点关注口腔病原体 Parvimonas micra 和 Fusobacter nucleatum。 在本研究中，使用 CRC 患者的粪便样本进行 16S rRNA 测序（n = 275）和无病理结果的对照 (n = 95)。我们发现，根据肿瘤位置和微卫星不稳定性 (MSI) 状态，微生物组成存在显着差异，P. micra、F. nucleatum 和消化链球菌在患者中含量更高MSI 肿瘤。此外，P. micra 和 F. nucleatum 与一系列 CRC 相关细菌（包括脆弱拟杆菌）以及其他口腔病原体（例如口腔假单胞菌和各种卟啉单胞菌）有关。该簇在对照组中明显不同，表明其与 CRC 存在潜在联系。我们的结果表明，CRC 患者体内几种 CRC 相关细菌的分布相似，这强调了在调查 CRC 机制的研究中考虑细菌种类同时存在的重要性。 CRC 的发展和进展。© 2024。作者。

Mounting evidence suggests a significant role of the gut microbiota in the development and progression of colorectal cancer (CRC). In particular, an over-representation of oral pathogens has been linked to CRC. The aim of this study was to further investigate the faecal microbial landscape of CRC patients, with a focus on the oral pathogens Parvimonas micra and Fusobacterium nucleatum.In this study, 16S rRNA sequencing was conducted using faecal samples from CRC patients (n = 275) and controls without pathological findings (n = 95).We discovered a significant difference in microbial composition depending on tumour location and microsatellite instability (MSI) status, with P. micra, F. nucleatum, and Peptostreptococcus stomatis found to be more abundant in patients with MSI tumours. Moreover, P. micra and F. nucleatum were associated with a cluster of CRC-related bacteria including Bacteroides fragilis as well as with other oral pathogens such as P. stomatis and various Porphyromonas species. This cluster was distinctly different in the control group, suggesting its potential linkage with CRC.Our results suggest a similar distribution of several CRC-associated bacteria within CRC patients, underscoring the importance of considering the concomitant presence of bacterial species in studies investigating the mechanisms of CRC development and progression.© 2024. The Author(s).