Parvimonas micra在结直肠癌患者口腔致病菌中形成独特的细菌网络

Parvimonas micra forms a distinct bacterial network with oral pathobionts in colorectal cancer patients

Journal of Translational Medicine

影响因子:7.5

分区:医学2区 Top / 医学：研究与实验2区

发表日期:2024 Oct 17

作者: Thyra Löwenmark, Linda Köhn, Therese Kellgren, William Rosenbaum, Vicky Bronnec, Anna Löfgren-Burström, Carl Zingmark, Pär Larsson, Michael Dahlberg, Bjoern O Schroeder, Sun Nyunt Wai, Ingrid Ljuslinder, Sofia Edin, Richard Palmqvist

DOI: 10.1186/s12967-024-05720-8

摘要

越来越多的证据表明，肠道微生物群在结直肠癌（CRC）的发生和发展中具有重要作用。特别是，口腔病原菌的过度表达与CRC密切相关。本研究旨在进一步探讨CRC患者的粪便微生物景观，重点关注口腔致病菌Parvimonas micra和Fusobacterium nucleatum。在本研究中，采用16S rRNA测序对CRC患者（n=275）和无病理发现的对照组（n=95）粪便样本进行了分析。我们发现微生物组成的显著差异依赖于肿瘤位置和微卫星不稳定性（MSI）状态，P. micra、F. nucleatum和Peptostreptococcus stomatis在MSI肿瘤患者中更为丰富。此外，P. micra和F. nucleatum与包括Bacteroides fragilis在内的多种CRC相关细菌簇以及其他口腔致病菌如P. stomatis和多种Porphyromonas属菌相关。该细菌簇在对照组中表现出明显不同，提示其可能与CRC有关。我们的结果表明，CRC患者中多种CRC相关菌的分布具有相似性，强调在研究CRC发生和发展的机制时考虑菌种共存的重要性。

Abstract

Mounting evidence suggests a significant role of the gut microbiota in the development and progression of colorectal cancer (CRC). In particular, an over-representation of oral pathogens has been linked to CRC. The aim of this study was to further investigate the faecal microbial landscape of CRC patients, with a focus on the oral pathogens Parvimonas micra and Fusobacterium nucleatum.In this study, 16S rRNA sequencing was conducted using faecal samples from CRC patients (n = 275) and controls without pathological findings (n = 95).We discovered a significant difference in microbial composition depending on tumour location and microsatellite instability (MSI) status, with P. micra, F. nucleatum, and Peptostreptococcus stomatis found to be more abundant in patients with MSI tumours. Moreover, P. micra and F. nucleatum were associated with a cluster of CRC-related bacteria including Bacteroides fragilis as well as with other oral pathogens such as P. stomatis and various Porphyromonas species. This cluster was distinctly different in the control group, suggesting its potential linkage with CRC.Our results suggest a similar distribution of several CRC-associated bacteria within CRC patients, underscoring the importance of considering the concomitant presence of bacterial species in studies investigating the mechanisms of CRC development and progression.