常用的抗菌剂由于过度使用或误用而不再有效。此外，许多药用植物提取物因其主要活性成分或次生代谢产物可以对抗传染病。目前的研究旨在评估 Launaea nudicaulis (LN) 叶提取物 (LE) 对不同多重耐药 (MDR) 铜绿假单胞菌 (P. aeruginosa) 分离株的生物活性。使用 GC-MS 分析了 Launaea nudicaulis (LN) 叶的乙酸乙酯提取物，鉴定出 27 种关键生物活性化合物。发现的主要成分如下：7-乙酰基-6-乙基-1,1,4,4-四甲基四氢萘、肉豆蔻酸异丙酯、硫代氨基甲酸、N,N-二甲基、S-1,3-二苯基-2-丁烯酯、hahnfett、环戊烷乙酸、3-氧代-2-戊基-甲酯、十六烷酸和三十烷。我们的研究表明，LN 叶是其他重要植物化学物质的丰富来源，包括酚酸、单宁、皂苷和类固醇。根据针对 Wi-38 正常细胞系的细胞毒性测定计算，裸茎 L. nudicaulis LE 的相对生物安全性是根据 262μg/mL 升高的抑制浓度 50 (IC50) 确定的。相反，所测试的提取物针对 MCF-7 和 Hep-G2 癌细胞系的 IC50 值记录较低，分别为 12.7 和 24.5μg/mL，反映了其对测试的癌细胞系的有效活性。在微生物学方面，对测试提取物敏感的铜绿假单胞菌分离株在孔扩散测定中表现出生长抑制区直径，范围为 11.34±0.47 至 26.67±0.47mm，抑制百分比 (PI) 值为 50-106.2%。反映其可接受的活动。此外，肉汤微量稀释测定法记录的最低抑菌浓度（MIC）和最低杀菌浓度（MBC）分别在15.625-1,000μg/mL和125-1,000μg/mL范围内。总之，裸茎 L. nudicaulis LE 对铜绿假单胞菌表现出良好的活性和高选择性。此外，该提取物在安全的低浓度下表现出天然抗癌活性，表明其作为活性成分未来研究的优秀候选者的潜力。版权所有 © 2024 Elkady、Badr、Hashem、Abdulrahman、Abdelaziz、Al-Askar、AbdElgayed 和 Hashem。

Commonly used antimicrobial agents are no longer effective due to their overuse or misuse. In addition, many medicinal plant extracts can combat infectious diseases due to their main active constituents or secondary metabolites. The current study aimed to assess the bioactivities of Launaea nudicaulis (LN) leaf extract (LE) against different multi-drug resistant (MDR) Pseudomonas aeruginosa (P. aeruginosa) isolates. The ethyl acetate extract of a Launaea nudicaulis (LN) leaf was analyzed using GC-MS, which identified 27 key bioactive compounds. The major constituents found were as follows: 7-acetyl-6-ethyl-1,1,4,4-tetramethyltetralin, isopropyl myristate, thiocarbamic acid, N,N-dimethyl, S-1,3-diphenyl-2-butenyl ester, hahnfett, cyclopentane acetic acid, 3-oxo-2-pentyl-, methyl ester, hexadecanoic acid, and dotriacontane. Our study demonstrated that the LN leaf was a rich source of other important phytochemicals, including phenolic acids, tannins, saponins, and steroids. The relative biosafety of the L. nudicaulis LE was determined from the elevated inhibitory concentration 50 (IC50) of 262 μg/mL, as calculated from the cytotoxicity assay against the Wi-38 normal cell line. Conversely, 12.7 and 24.5 μg/mL were the recorded low IC50 values for the tested extract against the MCF-7 and Hep-G2 cancerous cell lines, respectively, reflecting its potent activity against the tested cancerous cell lines. Microbiologically, the susceptible P. aeruginosa isolates to the tested extract showed a growth inhibition zone diameter, in the well diffusion assay, ranging from 11.34 ± 0.47 to 26.67 ± 0.47 mm, and a percent inhibition (PI) value of 50-106.2%, reflecting its acceptable activity. In addition, the broth microdilution assay recorded minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) in the ranges of 15.625-1,000 μg/mL and 125-1,000 μg/mL, respectively. In conclusion, the L. nudicaulis LE revealed showed promising activity and high selectivity against P. aeruginosa. Moreover, the extract exhibited natural anticancer activities with safe low concentrations, indicating its potential as a superior candidate for future studies of its active constituents.Copyright © 2024 Elkady, Badr, Hashem, Abdulrahman, Abdelaziz, Al-Askar, AbdElgayed and Hashem.