鼠乳杆菌gg和动物双歧杆菌亚种。乳酸BB-12通过调节伤口微环境促进受感染的伤口愈合
Lactobacillus rhamnosus GG and Bifidobacterium animalis subsp. lactis BB-12 promote infected wound healing via regulation of the wound microenvironment
影响因子:5.20000
分区:生物学2区 / 生物工程与应用微生物2区 微生物学3区
发表日期:2024 Oct
作者:
Zhe Yin, Yilin Wang, Xiaojuan Feng, Changqing Liu, Xiaoyang Guan, Shuyan Liu, Zhanyi Long, Zhonghua Miao, Fang He, Ruyue Cheng, Yanting Han, Ka Li
摘要
受感染的伤口会导致复杂的临床并发症和延迟的愈合，从而引起重大的全球公共卫生挑战。这项研究探讨了两种益生菌的局部应用，即乳糖乳杆菌GG（LGG）和动物双歧杆菌亚种的影响。乳酸BB-12，在感染伤口的微环境及其对伤口愈合的影响。每天将LGG和BB-12分别应用于金黄色葡萄球菌（金黄色葡萄球菌）感染的大鼠模型的皮肤伤口。两种益生菌都显着加速了伤口愈合，这是通过增强的肉芽组织形成和胶原蛋白沉积增加证明的，而BB-12显示出较高的功效。 LGG和BB-12都有效地抑制了嗜中性粒细胞的浸润，并降低了促炎性细胞因子肿瘤坏死因子-α（TNF-α）和白介素6（IL-6）的表达。值得注意的是，BB-12显着降低了IL-6水平，而LGG显着降低了TNF-α，转化生长因子-β（TGF-β）和血管内皮生长因子（VEGF）。另外，两种益生菌都促进了巨噬细胞极化，以抗炎M2表型。微生物群分析表明，LGG和BB-12显着降低了致病细菌（例如葡萄球菌和蛋白质）的丰度，并增加了有益细菌的比例（例如，Corynebacterium）。特别是，BB-12在减少葡萄球菌的丰度方面更有效，而LGG在促进Corynebacterium的生长方面表现出色。这些发现表明LGG和BB-12调节伤口微环境，增强伤口愈合并为受感染伤口的治疗提供了宝贵的见解。
Abstract
Infected wounds can result in complex clinical complications and delayed healing, presenting a significant global public health challenge. This study explored the effects of topical application of two probiotics, Lactobacillus rhamnosus GG (LGG) and Bifidobacterium animalis subsp. lactis BB-12, on the microenvironment of infected wounds and their impact on wound healing. LGG and BB-12 were applied separately and topically on the Staphylococcus aureus (S. aureus)-infected skin wounds of the rat model on a daily basis. Both probiotics significantly accelerated wound healing, demonstrated by enhanced granulation tissue formation and increased collagen deposition, with BB-12 showing superior efficacy. LGG and BB-12 both effectively inhibited neutrophil infiltration and decreased the expression of pro-inflammatory cytokines tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6). Notably, BB-12 markedly reduced IL-6 levels, while LGG significantly lowered TNF-α, transforming growth factor-β (TGF-β) and vascular endothelial growth factor (VEGF). Additionally, both probiotics promoted macrophage polarization towards the anti-inflammatory M2 phenotype. Microbiota analysis revealed that LGG and BB-12 significantly decreased the abundance of pathogenic bacteria (e.g. Staphylococcus and Proteus) and increased the proportion of beneficial bacteria (e.g. Corynebacterium). Particularly, BB-12 was more effective in reducing Staphylococcus abundance, whereas LGG excelled in promoting Corynebacterium growth. These findings suggest the ability of LGG and BB-12 to modulate the wound microenvironment, enhance wound healing and provide valuable insights for the management of infected wounds.