紫外线 (UV) 暴露主要通过破坏皮肤屏障功能来引发皮肤老化，导致皮肤干燥和皱纹形成。牡蛎水解物（OH）作为一种功能性食品，已被报道具有抗癌、抗氧化和抗细胞凋亡的作用。本研究调查了 OH 对 UVB 引起的 SKH1 无毛小鼠皮肤老化影响的潜在机制。小鼠每周暴露 3 次 UVB，同时喂食正常饮食或含有 OH 的饮食 10 周。此外，还进行了一项随机、双盲、安慰剂对照临床试验，研究 OH 对人体皮肤保湿的影响，以评估其功效和安全性。 UVB 暴露增加了皮肤老化参数；脱水、经表皮失水和肉眼可见的背部皮肤损伤。 OH显着降低了这些皮肤老化特征。组织学分析表明，OH 减少了 UVB 引起的皮肤表皮和真皮厚度以及胶原蛋白降解。 OH显着减少ROS的产生，抑制巨噬细胞活化和中性粒细胞浸润，并下调促炎细胞因子的产生。 OH 通过增加聚丝蛋白、水通道蛋白-3 和透明质酸合成酶的表达并促进皮肤损伤的恢复来改善皮肤屏障功能。重要的是，人体临床试验的结果表明，OH 可以改善皮肤的保湿性和完整性，并且没有副作用。总而言之，补充 OH 可通过抗氧化和抗炎特性改善皮肤损伤，并增强皮肤水合作用和屏障功能。 OH 对皮肤光老化具有治疗潜力。版权所有 © 2024。由 Elsevier Inc. 出版。

Ultraviolet (UV) exposure triggers skin aging primarily by disrupting skin barrier function, resulting in dry skin and wrinkle formation. Oyster hydrolysate (OH), as a functional food, has been reported for anti-cancer, anti-oxidant and anti-apoptotic effects. This study investigated the underlying mechanism of OH effect on UVB-induced skin aging in SKH1 hairless mice. Mice were exposed to UVB three times per week while they were fed with a normal diet or diet containing OH for 10 weeks. Additionally, a randomized, double-blind, and placebo-controlled clinical trial was performed to investigate the OH effect on human skin moisturizing to evaluate its efficacy and safety. UVB exposure increased parameters of skin aging; dehydration, transepidermal water loss, and macroscopic dorsal skin lesions. OH significantly reduced these features of skin aging. Histological analysis demonstrated that OH decreased skin epidermal and dermal thickness and collagen degradation induced by UVB. OH significantly reduced ROS production, suppressed macrophage activation and neutrophil infiltration, and downregulated pro-inflammatory cytokine production. OH improved skin barrier function by increasing the expression of filaggrin, aquaporin-3, and hyaluronic acid synthesis enzymes and promoting recovery from skin damage. Importantly, the results from a human clinical trial demonstrated that OH improved skin moisturization and integrity with no side effects. Taken together, OH supplementation ameliorates skin damage via anti-oxidant and anti-inflammatory properties and enhances skin hydration and barrier function. OH has a therapeutic potential to skin photoaging.Copyright © 2024. Published by Elsevier Inc.