百部 (Stemona tuberosa Lour) 的根已用于治疗肺结核、疥疮和湿疹。多糖是其主要生物活性成分之一。通过优化三相分配从马铃薯根中获得低分子量（1819Da）多糖（SPS2-A），并采用离子色谱柱纯化，并对其作用和机制进行了研究。结构分析表明，SPS2-A 含有阿拉伯糖、半乳糖 (Gal)、葡萄糖 (Glc)、木糖和甘露糖。 SPS2-A主链结构包含糖残基→4)-α-D-Glcp-(1→,→4)-α-D-Galp-(1→,和→4,6)-β-D-Galp- (1→，而侧链主要由α-D-Glcp-(1→连接到残基→4,6)-β-D-Galp-(1→。体外，SPS2- A 下调脂多糖诱导的 RAW264.7 巨噬细胞中白细胞介素 6 的表达 在体内，SPS2-A 显着下调支气管肺泡灌洗液和肺中髓过氧化物酶、白细胞介素 6、白细胞介素 1β 和肿瘤坏死因子 α 的表达。蛋白质印迹分析表明，SPS2-A 通过激活核因子 κB 通路，减轻脓毒症引起的急性肺损伤小鼠的肺部炎症。这些结果表明，SPS2-A 是治疗脓毒症的潜在抗炎候选药物。诱发急性肺损伤。版权所有 © 2024。由 Elsevier B.V. 出版。

The root of Stemona tuberosa Lour has been used to treat tuberculosis, scabies, and eczema. Polysaccharides are among its main bioactive ingredients. A low-molecular-weight (1819 Da) polysaccharide (SPS2-A) was obtained from the root of S. tuberosa Lour by optimizing three-phase partitioning, purified using an ion chromatography column, and its effects and mechanisms were investigated. Structural analysis revealed that SPS2-A contained arabinose, galactose (Gal), glucose (Glc), xylose, and mannose. The SPS2-A backbone structure comprised sugar residues →4)-α-D-Glcp-(1→, →4)-α-D-Galp-(1→, and →4,6)-β-D-Galp-(1→, while the side chain primarily comprised α-D-Glcp-(1 → connected to the O-6 position of the residue →4,6)-β-D-Galp-(1→. In vitro, SPS2-A downregulated the expression of interleukin-6 in lipopolysaccharide-induced RAW264.7 macrophages. In vivo, SPS2-A significantly downregulated the expression of myeloperoxidase, interleukin-6, interleukin-1β, and tumor necrosis factor-α in bronchoalveolar lavage fluid and lung tissue. Western blotting analysis indicated that SPS2-A reduced lung inflammation in mice with sepsis-induced acute lung injury by activating the nuclear factor κB pathway. These results suggest that SPS2-A is a potential anti-inflammatory candidate for the treatment of sepsis-induced acute lung injury.Copyright © 2024. Published by Elsevier B.V.