磁热疗与基因治疗相结合治疗乳腺癌。
Combination of magnetic hyperthermia and gene therapy for breast cancer.
发表日期：2024 Oct 19
作者：
Kubra Solak, Seyda Yildiz Arslan, Melek Acar, Fatma Turhan, Yagmur Unver, Ahmet Mavi
来源：
Cellular & Molecular Immunology
摘要：
这项研究提出了一种新型乳腺癌治疗模型，该模型使用磁场控制加热来触发癌细胞中的基因表达。我们创建了二氧化硅和胺修饰的超顺磁性纳米颗粒 (MSNP-NH2)，用于携带基因并在交流 (AC) 磁场下释放热量。热诱导表达质粒 (pHSP-Azu) 设计用于编码抗癌天青蛋白，并通过磁转染递送。当暴露于 75 µg/ml MSNP-NH2、3 µg DNA 和 PEI/DNA 比率（w: w）为 0.75 的 PEI 时，MCF-7 细胞表现出超过 93% 的细胞活力和 12% 的转染效率，与非致瘤细胞 (MCF-10 A)。磁热疗（MHT）通过热诱导增加天青蛋白的表达，从而以双重方式导致细胞死亡。 MHT 和热调节天青蛋白表达的结合会诱导细胞死亡，特别是癌细胞死亡，而对 MCF-10 A 细胞的影响可以忽略不计。所提出的策略清楚地表明，同时使用 MHT 和 MHT 诱导的天青蛋白基因表达可以选择性地靶向并杀死癌细胞，为癌症治疗提供有希望的方向。© 2024。作者，获得 Springer Science Business Media 独家许可，LLC，施普林格自然的一部分。
This study presented a novel breast cancer therapy model that uses magnetic field-controlled heating to trigger gene expression in cancer cells. We created silica- and amine-modified superparamagnetic nanoparticles (MSNP-NH2) to carry genes and release heat under an alternating current (AC) magnetic field. The heat-inducible expression plasmid (pHSP-Azu) was designed to encode anti-cancer azurin and was delivered by magnetofection. MCF-7 cells demonstrated over 93% cell viability and 12% transfection efficiency when exposed to 75 µg/ml of MSNP-NH2, 3 µg of DNA, and PEI at a 0.75 PEI/DNA ratio (w: w), unlike non-tumorigenic cells (MCF-10 A). Magnetic hyperthermia (MHT) increased azurin expression by heat induction, leading to cell death in dual ways. The combination of MHT and heat-regulated azurin expression induced cell death, specifically in cancer cells, while having negligible effects on MCF-10 A cells. The proposed strategy clearly shows that simultaneous use of MHT and MHT-induced azurin gene expression may selectively target and kill cancer cells, offering a promising direction for cancer therapy.© 2024. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.