作为一种新的细胞死亡形式，铜凋亡是由铜与三羧酸循环中的脂化蛋白质结合引起的。据我们所知，尚未有研究全面分析铜凋亡相关基因与乳腺癌的关系。分析乳腺癌患者铜凋亡相关基因的表达、预后价值、突变、化疗敏感性和免疫浸润情况，PPI网络构建了这些基因，并根据这些基因进行了富集分析。使用TIMER、UALCAN、Kaplan-Meier绘图仪、Human Protein Atlas、cBioPortal、STRING、GeneMANIA、DAVID和R程序v4.0.3完成上述分析。与正常乳腺组织相比，FDX1、LIAS、LIPT1、DLD、DLAT乳腺癌组织中PDHA1、MTF1和GLS表达下调，而CDKN2A表达上调。 FDX1、LIAS、DLD、DLAT、MTF1、GLS 和 CDKN2A 的高表达与良好的总生存期相关。铜中毒相关基因在乳腺癌中表现出较高的改变率（51.3%），导致较差的临床结果。 FDX1、LIPT1、DLD、DLAT、PDHA1、PDHB、MTF1、GLS和CDKN2A的表达水平与乳腺癌中1个或多个免疫细胞浸润呈正相关。 B细胞、CD4 T细胞、CD8 T细胞和树突状细胞浸润水平较高的患者10年生存率较高。本研究通过生物信息学分析全面探讨了铜凋亡与乳腺癌之间的关系。我们发现铜凋亡相关基因在乳腺癌组织中普遍低表达。 FDX1作为铜凋亡的关键基因，其高表达与乳腺癌患者良好的预后相关；因此，它可能是一个潜在的预后标志物。此外，与铜凋亡相关的基因与乳腺癌的免疫浸润有关，这种关系影响乳腺癌的预后。版权所有 © 2024 作者。由 Wolters Kluwer Health, Inc. 出版

As a novel cell death form, cuproptosis results from copper combining with lipidated proteins in the tricarboxylic acid cycle. To the best of our knowledge no study has yet comprehensively analyzed the relationship between cuproptosis-related genes and breast cancer.The expression, prognostic value, mutations, chemosensitivity, and immune infiltration of cuproptosis-related genes in breast carcinoma patients were analyzed, PPI networks were constructed, and enrichment analyses were performed based on these genes. TIMER, UALCAN, Kaplan-Meier plotter, Human Protein Atlas, cBioPortal, STRING, GeneMANIA, DAVID, and R program v4.0.3 were used to accomplish the analyses above.Compared to normal breast tissues, FDX1, LIAS, LIPT1, DLD, DLAT, PDHA1, MTF1, and GLS were down-regulated in breast cancer tissues, while CDKN2A was up-regulated. High expression of FDX1, LIAS, DLD, DLAT, MTF1, GLS, and CDKN2A were associated with favorable overall survival. Cuproptosis-related genes showed a high alteration rate (51.3%) in breast cancer, contributing to worse clinical outcomes. The expression levels of FDX1, LIPT1, DLD, DLAT, PDHA1, PDHB, MTF1, GLS, and CDKN2A were associated positively with 1 or more immune cell infiltrations in breast cancer. Patients with high levels of B cell, CD4+ T cell, CD8+ T cell, and dendritic cell infiltration had a higher survival rate at 10 years.This study comprehensively investigated relationships between cuproptosis and breast cancer by bioinformatic analyses. We found that cuproptosis-related genes were generally lowly expressed in breast carcinoma tissue. As the critical gene of cuproptosis, high expression of FDX1 was related to favorable prognoses in breast cancer patients; thus, it might be a potential prognostic marker. Moreover, genes associated with cuproptosis were linked to immune infiltration in breast cancer and this relationship affected the prognosis of breast cancer.Copyright © 2024 the Author(s). Published by Wolters Kluwer Health, Inc.