槐花是一种传统中药材和保健茶，由豆科植物槐的干燥花和花蕾组成。本研究评估了槐花提取物 (FSFE) 对人肝癌细胞系 HepG2 的体外抗癌功效，并与其对正常人肝 L02 细胞的作用进行了比较。使用 3-(4,5-二甲基-2-噻唑基)-2,5-二苯基溴化四唑 (MTT) 测定法评估细胞活力，以评估果皮黄酮提取物 (FSFE) 对细胞增殖的影响。结果表明，FSFE显着抑制HepG2肝癌细胞的增殖，抑制浓度（IC50）为117.98μg/mL，而对正常肝L02细胞的影响极小。 HPLC 分析确定芦丁和槲皮素是 FSFE 的成分，两者均具有抗氧化特性。槐花中的黄酮类化合物对肝癌细胞表现出有效的抑制作用，表明其作为天然抗癌剂的潜力。这些发现支持对这些化合物的治疗应用的持续开发和研究。版权所有 © 2024。由 Elsevier B.V. 出版。

Flos Sophorae, a traditional Chinese medicinal herb and health tea, consists of the dried flowers and buds of the Sophora japonica L. (Leguminosae). This study assesses the in vitro anticancer efficacy of Flos Sophorae flower extract (FSFE) against the human hepatocarcinoma cell line HepG2, juxtaposed with its effects on normal human liver L02 cells. Cell viability was assessed using the 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl tetrazolium bromide (MTT) assay to evaluate the effects of fruit skin flavonoid extract (FSFE) on cellular proliferation. The results indicated that FSFE significantly inhibited the proliferation of HepG2 liver cancer cells, with an inhibitory concentration (IC50) of 117.98 μg/mL, while having minimal effects on normal liver L02 cells. HPLC analysis identified rutin and quercetin as components of FSFE, both recognized for their antioxidant properties. The flavonoids in Flos Sophorae exhibit potent inhibitory effects on liver cancer cells, indicating potential as a natural anticancer agent. The findings support the continued development and research into the therapeutic applications of these compounds.Copyright © 2024. Published by Elsevier B.V.