旨在检查维生素 C 和氯硝柳胺一起使用对甲氨蝶呤引起的肝损伤的影响。该研究于 11 月起在伊拉克巴格达 Mustansiriya 大学医学院药理学系和伊拉克癌症和医学遗传学研究中心进行2020 年至 2021 年 7 月，由被随机分配到 5 组的白化小鼠组成。第1组包括对照组，第2至5组分别接受甲氨蝶呤、氯硝柳胺70mg/kg/天、维生素C 100mg/kg/天以及氯硝柳胺和维生素C的组合。第3、4和5组的小鼠还接受腹腔注射甲氨蝶呤20mg/kg以诱导肝毒性。注射48小时后，在氯仿麻醉下处死小鼠。抽取心脏血样进行生化检查。肝脏洗净后分成两部分；一部分进行组织学检查，另一部分保存在10%福尔马林中用于组织病理学分析。使用SPSS 16分析数据。35只小鼠中，5组各有7只(20%)。总体年龄在 9-12 周之间，体重在 18-38 克之间。结果显示，氯硝柳胺和维生素 C 分别产生显着的肝脏保护作用（p 值 <0.05），表现为 ALP、ALT 和 LDH 的降低，而氯硝柳胺和维生素 C 的组合则没有表现出相加作用 (p> 0.05) 增强肝功能。发现单独使用氯硝柳胺比与维生素 C 联用更能治疗甲氨蝶呤引起的肝损伤。

To examine the effect of using vitamin C and niclosamide together on liver damage caused by methotrexate.The study was conducted at the Pharmacology Department and the Iraqi Center for Cancer and Medical Genetics Research, College of Medicine, Mustansiriya University, Baghdad, Iraq, from November 2020 to July 2021, and comprised albino mice who were randomly assigned to 5 groups. Group 1 comprised controls, groups 2 to 5 was received methotrexate, niclosamide 70mg/kg/day, vitamin C 100mg/kg/day, and a combination of niclosamide and vitamin C, respectively. Mice in groups 3, 4 and 5 also received an intraperitoneal injection of methotrexate 20mg/kg to induce hepatotoxicity. After 48 hours of the injection, the mice were sacrificed under chloroform anaesthesia. Cardiac blood samples were drawn for biochemical examination. The liver, after being washed, was divided into two parts; one part was taken for histological examination, and the other was preserved in formalin 10% for histopathological analyses. Data was analysed using SPSS 16.Of the 35 mice, there were 7(20%) in each of the 5 groups. The overall age ranged between 9-12 weeks and weight between 18-38gm. The results show significant hepatoprotection ( p-value <0.05) produced by both niclosamide and Vitamin C separately, reflected by a decrease in ALP, ALT, and LDH, while the combination of (niclosamide and Vitamin C) showed no additive effect (p>0.05) on enhancement of liver function.Niclosamide alone was found to be superior than in combination with vitamin C for treating methotrexate-induced liver damage.